Department of Forest and Soil Sciences, Boku, University of Natural Resources and Life Sciences, Vienna, Austria.
Division of Nuclear Techniques in Food and Agriculture, Insect Pest Control Subprogramme, Joint FAO/IAEA, Vienna, Austria.
PLoS One. 2019 Dec 31;14(12):e0226582. doi: 10.1371/journal.pone.0226582. eCollection 2019.
Treating insects with a lower oxygen atmosphere before and during exposure to radiation can mitigate some of the negative physiological effects due to the irradiation. The irradiation of pupae under oxygen-reduced environment such as hypoxia or anoxia is routinely used in the sterile insect technique (SIT) of some tephritid species as it provides radiological protection. This treatment allows to have the sterile pupae already in sealed containers facilitating the shipment. SIT is an environment friendly control tactic that could be used to manage populations of Drosophila suzukii in confined areas such as greenhouses. The objectives of this study were to assess the effect of irradiation on the reproductive sterility in D. suzukii males and females under low-oxygen atmosphere (hypoxia) and atmosphere conditions (normoxia). Additionally, we assessed the differences in radiological sensitivity of pupae treated under hypoxia and normoxia conditions. Finally, the effect on emergence rate and flight ability of the irradiated D. suzukii adults exposed to doses that induced >99% of sterility were assessed. Pupae needed a 220 Gy irradiation dose to achieve >99% of egg hatch sterility in males irrespective of the atmosphere condition. For females the same level of sterility was achieved already at 75 Gy and 90 Gy for the normoxia and hypoxia treatments, respectively. Radiation exposure at 170 and 220 Gy under the two atmosphere treatments did not have any effect on the emergence rate and flight ability of D. suzukii males and females. Therefore, hypoxia conditions can be used as part of an area-wide insect pest management program applying SIT to facilitate the protocols of packing, irradiation and shipment of sterile D. suzukii pupae.
在暴露于辐射之前和期间，用低氧环境处理昆虫可以减轻因辐射引起的一些负面生理效应。在缺氧或缺氧等减氧环境下辐照蛹，在一些桃小食心虫物种的无菌昆虫技术（SIT）中是常规做法，因为它提供放射防护。这种处理方法可以使无菌蛹已经在密封的容器中，便于运输。SIT 是一种环保的控制策略，可用于管理温室等封闭区域中的果蝇的种群。本研究的目的是评估在低氧（缺氧）和大气条件（常氧）下辐照对 D. suzukii 雄性和雌性生殖不育的影响。此外，我们评估了在缺氧和常氧条件下处理的蛹的放射敏感性差异。最后，评估了暴露于诱导 >99%不育的剂量下的辐照 D. suzukii 成虫的出蛹率和飞行能力的影响。无论大气条件如何，雄性蛹都需要 220 Gy 的辐照剂量才能达到 >99%的卵孵化不育。对于雌性，在常氧和缺氧处理下，分别达到 75 Gy 和 90 Gy 就可以达到相同水平的不育。在两种大气处理下，170 和 220 Gy 的辐射暴露对 D. suzukii 雄性和雌性的出蛹率和飞行能力没有任何影响。因此，缺氧条件可以作为应用 SIT 进行区域害虫综合治理计划的一部分，以促进无菌 D. suzukii 蛹的包装、辐照和运输协议。
