Askarian Fatemeh, Lapek John D, Dongre Mitesh, Tsai Chih-Ming, Kumaraswamy Monika, Kousha Armin, Valderrama J Andrés, Ludviksen Judith A, Cavanagh Jorunn P, Uchiyama Satoshi, Mollnes Tom E, Gonzalez David J, Wai Sun N, Nizet Victor, Johannessen Mona
Research Group of Host Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, UiT - The Arctic University of Norway, Tromsø, Norway.
Division of Host-Microbe Systems and Therapeutics, Department of Pediatrics, University of California, San Diego, La Jolla, CA, United States.
Front Microbiol. 2018 Feb 20;9:262. doi: 10.3389/fmicb.2018.00262. eCollection 2018.
produces membrane-derived vesicles (MVs), which share functional properties to outer membrane vesicles. Atomic force microscopy revealed that -derived MVs are associated with the bacterial surface or released into the surrounding environment depending on bacterial growth conditions. By using a comparative proteomic approach, a total of 131 and 617 proteins were identified in MVs isolated from grown in Luria-Bertani and brain-heart infusion broth, respectively. Purified MVs derived from the bacteria grown in either media induced comparable levels of cytotoxicity and neutrophil-activation. Administration of exogenous MVs increased the resistance of to killing by whole blood or purified human neutrophils and increased survival . Finally, immunization of mice with -derived MVs induced production of IgM, total IgG, IgG1, IgG2a, and IgG2b resulting in protection against subcutaneous and systemic infection. Collectively, our results suggest MVs can influence bacterial-host interactions during systemic infections and provide protective immunity in murine models of infection.
产生膜衍生囊泡（MVs），其与外膜囊泡具有共同的功能特性。原子力显微镜显示，根据细菌生长条件，衍生的MVs与细菌表面相关或释放到周围环境中。通过使用比较蛋白质组学方法，分别在从生长于Luria-Bertani和脑心浸液肉汤中的分离出的MVs中鉴定出总共131种和617种蛋白质。从在两种培养基中生长的细菌衍生的纯化MVs诱导了相当水平的细胞毒性和中性粒细胞活化。外源性MVs的施用增加了对全血或纯化的人中性粒细胞杀伤的抵抗力，并提高了存活率。最后，用衍生的MVs免疫小鼠诱导了IgM、总IgG、IgG1、IgG2a和IgG2b的产生，从而提供了针对皮下和全身感染的保护。总体而言，我们的结果表明MVs可在全身感染期间影响细菌与宿主的相互作用，并在小鼠感染模型中提供保护性免疫。
