Ali Waqas, Habib Mudasser, Khan Rai Shafqat Ali, Zia Muhammad Ashir, Farooq Muhammad, Sajid Sanaullah, Shah Muhammad Salah Ud Din
Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan.
Arch Virol. 2018 Jul;163(7):1733-1743. doi: 10.1007/s00705-018-3775-0. Epub 2018 Mar 7.
This study reports the molecular characterization of foot-and-mouth disease virus (FMDV) in the provinces of Punjab and Sindh, Pakistan during 2014-17. FMDV genome was detected in 42 and 41 out of 46 samples (epithelial tissue and saliva) by reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. Sequences of the complete VP1 coding region of the samples (n = 33) was achieved showing that 10, 4 and 19 samples belonged to serotype O, A and Asia1 respectively. Phylogenetic analysis of serotype O revealed that at least one novel sublineage within the ME-SA topotype is circulating in the region, named here as PAK-14. This sublineage showed similarity with the viruses circulating in Turkey and Pakistan during 2010 indicating that viruses circulating in these countries have common origin. Analysis of serotype A viruses revealed a new lineage is circulating in the region, reported here as A-PAK14 showing close identity with the strain prevalent in Pakistan during 2007. Circulation of these new linages in the region shows continuous evolution of the viruses. Two of the undisclosed serotype A sublineages within the Iran-05 lineage were also found circulating in the region. In addition, molecular investigation of the VP1 coding region sequences of serotype Asia1 strains revealed that they belong to Group-VII (Sindh-08). Interestingly some of the serotype Asia1 isolates (n = 6) showed 99.9% similarity (among themselves) although they were collected from different districts more than 100 Km apart from one another. This unusual conservation among serotype Asia1 over long distances can be explored by studying the role of wild animals, slaughter houses and milk collection centres in the spread the disease.
本研究报告了2014 - 2017年期间巴基斯坦旁遮普省和信德省口蹄疫病毒（FMDV）的分子特征。分别通过逆转录环介导等温扩增（RT-LAMP）和逆转录聚合酶链反应（RT-PCR）在46份样本（上皮组织和唾液）中的42份和41份中检测到FMDV基因组。获得了样本（n = 33）完整VP1编码区的序列，结果显示10份、4份和19份样本分别属于O型、A型和亚洲1型。O型的系统发育分析表明，中东 - 南非（ME-SA）拓扑型内至少有一个新的亚谱系在该地区传播，在此命名为PAK-14。该亚谱系与2010年在土耳其和巴基斯坦传播的病毒相似，表明在这些国家传播的病毒有共同起源。A型病毒分析显示该地区有一个新的谱系在传播，在此报告为A-PAK14，与2007年在巴基斯坦流行的毒株密切相关。这些新谱系在该地区的传播表明病毒在持续进化。还发现伊朗 - 05谱系内两个未公开的A型亚谱系也在该地区传播。此外，对亚洲1型毒株VP1编码区序列的分子研究表明它们属于第七组（信德 - 08）。有趣的是，一些亚洲1型分离株（n = 6）尽管彼此采集于相距100多公里的不同地区，但它们之间显示出99.9%的相似性。通过研究野生动物、屠宰场和牛奶收集中心在疾病传播中的作用，可以探究亚洲1型在远距离的这种异常保守性。
