a Department of Microbiology and Parasitology, College of Veterinary Medicine , King Faisal University , Al-Hasa, Saudi Arabia.
b Department of Virology, Faculty of Veterinary Medicine , Kafrelsheikh University , Kafelsheikh, Egypt.
Vet Q. 2018 Dec;38(1):88-98. doi: 10.1080/01652176.2018.1539568.
Foot-and-mouth disease virus (FMDV) is a highly contagious viral infection of large ruminants. Despite the massive application of vaccines against FMDV, several outbreaks are still being reported in Africa and Asia.
To perform molecular characterization of FMDV in an outbreak among a cattle herd Saudi Arabia in 2016. This herd had been vaccinated with a polyvalent FMDV vaccine.
To investigate this outbreak, we collected specimens from 77 animals showing typical clinical signs of FMDV. Specimens including sera, nasal swabs, and tissues (tongue, coronary bands, hooves, and hearts) were collected. We tested the collected cattle sera for the presence of FMDV antibodies with commercial ELISA kits. In addition, we tested the swabs for the presence of the most common FMDV strains (O, A, Asia-1 and SAT-2) with RT-PCR using serotype-specific oligonucleotides.
Serology showed that 22% of the tested sera were positive. Molecular testing of the examined swabs confirmed that 24% of the tested animals were positive. Our sequencing analysis confirmed that the circulating strains of FMDV belonged to FMDV serotype O. The phylogenetic tree based on the FMDV-VP-1 gene revealed high nucleotide identity between the circulating strains and the Bangladesh strain (99%). These strains were distinct (shared 89% nucleotide identity) from the FMDV-O strains used for the preparation of the vaccine administered to the animals in this herd. Moreover, they had 7% nucleotide difference between the FMDV-O strains reported in Saudi Arabian in 2013.
More in-depth molecular characterization of these FMDV strains is warranted.
口蹄疫病毒（FMDV）是一种高度传染性的大型反刍动物病毒感染。尽管大规模应用了针对 FMDV 的疫苗，但在非洲和亚洲仍有几处暴发。
对 2016 年沙特阿拉伯的牛群暴发 FMDV 进行分子特征描述。该牛群已接种了多价 FMDV 疫苗。
为了调查这次暴发，我们从 77 头表现出口蹄疫病毒典型临床症状的动物中采集了样本。采集了包括血清、鼻拭子和组织（舌、冠状带、蹄和心脏）的标本。我们使用商业 ELISA 试剂盒检测采集的牛血清中是否存在 FMDV 抗体。此外，我们使用针对最常见的 FMDV 株（O、A、Asia-1 和 SAT-2）的 RT-PCR 检测拭子中是否存在 FMDV。
血清学检测显示，22%的检测血清呈阳性。对检查拭子的分子检测证实，24%的受检动物呈阳性。我们的测序分析证实，循环的 FMDV 株属于 FMDV 血清型 O。基于 FMDV-VP-1 基因的系统进化树显示，循环株与孟加拉国株的核苷酸同源性很高（99%）。这些与用于接种该牛群动物的疫苗制备的 FMDV-O 株不同（共享 89%核苷酸同一性）。此外，它们与 2013 年沙特阿拉伯报告的 FMDV-O 株之间存在 7%的核苷酸差异。
有必要对这些 FMDV 株进行更深入的分子特征描述。
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