Ullah A, Jamal S M, Romey A, Gorna K, Kakar M A, Abbas F, Ahmad J, Zientara S, Bakkali Kassimi L
Center for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan.
Department of Biotechnology and Informatics, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology, Engineering and Management Sciences, Beleli, Quetta.
Transbound Emerg Dis. 2017 Oct;64(5):1569-1578. doi: 10.1111/tbed.12548. Epub 2016 Aug 2.
This study reports characterization of foot-and-mouth disease virus (FMDV) in samples collected from Balochistan, Pakistan. FMDV was detected by pan-FMDV real-time RT-PCR in 31 samples (epithelial and oral swabs) collected in 2011 from clinical suspect cases. Of these, 29 samples were serotyped by serotype-specific real-time RT-PCR assays and were confirmed by sequencing the VP1 coding region. Sixteen samples were found positive for serotype A and eight for serotype Asia-1, whereas five samples were found positive for both serotypes A and Asia-1. Two serotype A positive samples were found positive for two different strains of serotype A FMDV each. Phylogenetic analyses of serotype A FMDVs showed circulation of at least three different sublineages within the A-Iran05 lineage. These included two earlier reported sublineages, A-Iran05 and A-Iran05 , and a new sublineage, designated here as A-Iran05 . This shows that viruses belonging to the A-Iran05 lineage are continuously evolving in the region. Viruses belonging to the A-Iran05 sublineage showed close identity with the viruses circulating in 2009 in Pakistan and Afghanistan. However, viruses belonging to the A-Iran05 detected in Balochistan, Pakistan, showed close identity with the viruses circulating in Kyrgyzstan, Iran and Kazakhstan in 2011 and 2012, showing that viruses responsible for outbreak in these countries have a common origin. Serotype Asia-1 FMDVs reported in this study all belonged to the earlier reported Group-VII (Sindh-08), which is currently a dominant strain in the West Eurasian region. Detection of two different serotypes of FMDV or/and two different strains of the same serotype in one animal/sample shows complexity in occurrence of FMD in the region.
本研究报告了从巴基斯坦俾路支省采集的样本中口蹄疫病毒（FMDV）的特征。2011年从临床疑似病例采集的31份样本（上皮和口腔拭子）通过泛口蹄疫病毒实时逆转录聚合酶链反应（pan-FMDV real-time RT-PCR）检测到口蹄疫病毒。其中，29份样本通过血清型特异性实时逆转录聚合酶链反应检测进行血清分型，并通过对VP1编码区进行测序得到确认。16份样本血清型A呈阳性，8份样本血清型Asia-1呈阳性，而5份样本血清型A和Asia-1均呈阳性。两份血清型A阳性样本分别对两种不同的血清型A口蹄疫病毒株呈阳性。血清型A口蹄疫病毒的系统发育分析表明，在A-Iran05谱系内至少有三个不同的亚谱系在传播。其中包括两个先前报道的亚谱系，A-Iran05和A-Iran05，以及一个新的亚谱系，在此指定为A-Iran05。这表明属于A-Iran05谱系的病毒在该地区不断进化。属于A-Iran05亚谱系的病毒与2009年在巴基斯坦和阿富汗传播的病毒具有密切的同一性。然而，在巴基斯坦俾路支省检测到的属于A-Iran05的病毒与2011年和2012年在吉尔吉斯斯坦、伊朗和哈萨克斯坦传播的病毒具有密切的同一性，表明这些国家爆发疫情的病毒有共同的起源。本研究报告的血清型Asia-1口蹄疫病毒均属于先前报道的VII组（信德-08），该组目前是西亚地区的优势毒株。在一只动物/样本中检测到两种不同血清型的口蹄疫病毒或/和同一血清型的两种不同毒株，表明该地区口蹄疫的发生情况较为复杂。
