Department of Periodontology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
Department of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences Vinca, University of Belgrade, Belgrade, Serbia.
J Clin Periodontol. 2018 Jun;45(6):663-671. doi: 10.1111/jcpe.12888. Epub 2018 May 15.
Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus (DM) type 2. In addition, circulatory microRNAs (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR-146a and miR-155 and superoxide dismutase (SOD) activity in gingival crevicular fluid (GCF) of periodontitis patients with (CPDM) and without (CP) DM type 2 as well as in periodontally healthy, control groups (PHDM and PH, respectively).
miR modulation was analysed using quantitative real-time PCR while SOD activity was measured spectrophotometrically.
The upregulation of miR-146a and miR-155 was observed in CP and CPDM patients' baseline, while the levels decreased after 6 weeks of the non-surgical therapy to the levels comparable to PH and PHDM, respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR-146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR-146a and miR-155 were significantly associated with periodontitis when adjusting for age and gender.
miR-146a and miR-155 may be considered as possible novel biomarkers for periodontitis in non-diabetic and type 2 diabetic patients.
最近的研究表明，表观遗传机制在牙周炎和 2 型糖尿病（DM）等多因素疾病的发展中起着关键作用。此外，循环 microRNAs（miRs）已成为各种疾病的新型生物标志物。本研究旨在探讨牙周炎伴或不伴 2 型 DM 患者（CPDM 和 CP）及牙周健康对照者（PHDM 和 PH）龈沟液（GCF）中 miR-146a 和 miR-155 的水平及超氧化物歧化酶（SOD）活性。
采用实时定量 PCR 分析 miR 调节，分光光度法测量 SOD 活性。
CP 和 CPDM 患者基线时 miR-146a 和 miR-155 上调，而非手术治疗 6 周后降至与 PH 和 PHDM 相当的水平。miR 的表达水平与 SOD 活性呈正相关。PHDM 患者的 miR-146a 水平高于 PH 患者。多变量分析显示，调整年龄和性别后，miR-146a 和 miR-155 水平与牙周炎显著相关。
miR-146a 和 miR-155 可作为非糖尿病和 2 型糖尿病患者牙周炎的潜在新型生物标志物。
