Department of Chemistry, University of Bath, BA2 7AY, Bath, UK.
Scapa Healthcare, Hilldrop Lane, Ramsbury, Marlborough, SN8 2RB, UK.
J Biomed Mater Res B Appl Biomater. 2019 Jan;107(1):129-137. doi: 10.1002/jbm.b.34103. Epub 2018 Mar 8.
It is becoming increasingly accepted that to understand and model the bacterial colonization and infection of abiotic surfaces requires the use of a biofilm model. There are many bacterial colonizations by at least two primary species, however this is difficult to model in vitro. This study reports the development of a simple mixed-species biofilm model using strains of two clinically significant bacteria: Staphylococcus aureus and Pseudomonas aeruginosa grown on nanoporous polycarbonate membranes on nutrient agar support. Scanning electron microscopy revealed the complex biofilm characteristics of two bacteria blending in extensive extracellular matrices. Using a prototype wound dressing which detects cytolytic virulence factors, the virulence secretion of 30 single and 40 mixed-species biofilms was tested. P. aeruginosa was seen to out-compete S. aureus, resulting in a biofilm with P. aeruginosa dominating. In situ growth of mixed-species biofilm under prototype dressings showed a real-time correlation between the viable biofilm population and their associated virulence factors, as seen by dressing fluorescent assay. This paper aims to provide a protocol for scientists working in the field of device related infection to create mixed-species biofilms and demonstrate that such biofilms are persistently more virulent in real infections. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 129-137, 2019.
越来越多的人认为，要理解和模拟细菌对非生物表面的定殖和感染，就需要使用生物膜模型。至少有两种主要物种会进行许多细菌定殖，但这在体外很难模拟。本研究报告了一种使用两种临床上重要的细菌（金黄色葡萄球菌和铜绿假单胞菌）菌株在营养琼脂支持的纳米多孔聚碳酸酯膜上生长的简单混合物种生物膜模型的开发。扫描电子显微镜揭示了两种细菌在广泛的细胞外基质中混合的复杂生物膜特征。使用原型伤口敷料来检测细胞毒性毒力因子，测试了 30 个单种和 40 个混合物种生物膜的毒力分泌。结果发现铜绿假单胞菌能够胜过金黄色葡萄球菌，导致生物膜中铜绿假单胞菌占主导地位。在原型敷料下混合物种生物膜的原位生长显示了活生物膜种群与其相关毒力因子之间的实时相关性，这可以通过敷料荧光分析来观察到。本文旨在为从事与器械相关感染领域的科学家提供一种创建混合物种生物膜的方案，并证明此类生物膜在实际感染中具有持续更高的毒力。© 2018 威利父子公司。J 生物医学材料研究部分 B：应用生物材料，107B：129-137，2019 年。
