Marumo F, Masuda T, Ando K
Biochem Biophys Res Commun. 1987 Mar 30;143(3):813-8. doi: 10.1016/0006-291x(87)90321-4.
Using a highly sensitive and specific radioimmunoassay (RIA) for detection of the atrial natriuretic peptide (ANP), the presence of alpha-human ANP (alpha-hANP) in human cerebrospinal fluid (CSF) was confirmed. Its concentration in CSF, 3.6 +/- 2.3 pg/ml, n = 16, mean +/- SD, was remarkably lower than that in the plasma (161.8 +/- 157.4, p less than 0.0001). The regression coefficient between these concentrations was 0.320 (p = ns). Gel permeation chromatography conducted in conjunction with RIA indicated ANP in CSF to be eluted at the position of a low molecular weight form corresponding to alpha-hANP. No high molecular weight form could be detected. But in the plasma, both low and high molecular forms were found to be present. It is thus evident that ANP is present in human CSF and its origin may possibly be the brain and not the atrium.
采用高灵敏度和特异性的放射免疫分析法（RIA）检测心房利钠肽（ANP），证实在人脑脊液（CSF）中存在α-人ANP（α-hANP）。其在脑脊液中的浓度为3.6±2.3 pg/ml，n = 16，均值±标准差，显著低于血浆中的浓度（161.8±157.4，p<0.0001）。这些浓度之间的回归系数为0.320（p =无显著性差异）。与RIA联合进行的凝胶渗透色谱表明，脑脊液中的ANP在对应于α-hANP的低分子量形式位置被洗脱。未检测到高分子量形式。但在血浆中，发现同时存在低分子量和高分子量形式。因此，很明显人脑脊液中存在ANP，其来源可能是脑而非心房。
