College of Animal Science, Guangdong Provincial Key Laboratory of Animal Nutrition Control, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2018 Mar 9;19(3):783. doi: 10.3390/ijms19030783.
Adipose tissue plays an important role in energy metabolism. Adipose dysfunction is closely related to obesity and type II diabetes. Glucose uptake is the key step for fat synthesis in adipocyte. miRNAs have been proven to play a crucial role in adipocyte differentiation, adipogenesis and glucose homeostasis. In this paper, we firstly reported that miR-146b decreased glucose consumption by up-regulating miR-146b in a porcine primary adipocyte model, while the inhibitor of endogenous miR-146b rescued the reduction. Then, miR-146b was predicated to target by bioinformatics analysis, and a dual-luciferase reporter assay validated this predication. Western blot analyses indicated both and glucose transporter type 4 (GLUT4) were down-regulated by miR-146b overexpression. Our study demonstrated that miR-146b regulated glucose homeostasis in porcine primary pre-adipocyte by targeting , and provided new understandings on regulations of lipogenesis by miRNAs.
脂肪组织在能量代谢中起着重要作用。脂肪功能障碍与肥胖和 II 型糖尿病密切相关。葡萄糖摄取是脂肪合成细胞中脂肪合成的关键步骤。miRNA 已被证明在脂肪细胞分化、脂肪生成和葡萄糖稳态中发挥关键作用。在本文中，我们首先报道了 miR-146b 通过上调猪原代脂肪细胞模型中的 miR-146b 来降低葡萄糖消耗，而内源性 miR-146b 的抑制剂则挽救了这种降低。然后，通过生物信息学分析预测 miR-146b 靶向 ，双荧光素酶报告基因实验验证了这一预测。Western blot 分析表明，miR-146b 过表达下调了 和葡萄糖转运蛋白 4 (GLUT4)。我们的研究表明，miR-146b 通过靶向 调节猪原代前脂肪细胞的葡萄糖稳态，为 miRNA 对脂肪生成的调控提供了新的认识。
