Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, 730070 Lanzhou, China.
Talanta. 2012 Nov 15;101:374-81. doi: 10.1016/j.talanta.2012.09.044. Epub 2012 Sep 28.
A novel molecularly imprinted (MIP) impedimetric sensor was promoted for selective detecting melamine (MEL). The Au electrode modified with MIP poly (2-mercaptobenzimidazole) (PMBI) was prepared by electrochemical polymerization of 2-mercaptobenzimidazole (2-MBI) with cyclic voltammetry (CV) in the presence of template molecule MEL. The surface morphology and structure of MIP PMBI are characterized by atomic force microscopy (AFM), infrared spectra (IR), electrochemical impedance spectroscopy (EIS), and CV. The main driving force of recognition is the π-donor-acceptor interaction between MEL and PMBI. The imprinted electrode could avoid the interference successfully. In addition, a linear response curve was obtained from 1.0×10(-8) M to 5.0×10(-5) M, with the detection limit of 3.0×10(-9) M. The sensor exhibits remarkable advantages, such as higher sensitivity, wider linear range and lower detection limit. The effective method has a potential application to monitor nonelectrochemically active substances in food analysis in the future.
一种新型的分子印迹（MIP）电化学传感器被提出用于选择性检测三聚氰胺（MEL）。通过循环伏安法（CV）在模板分子 MEL 的存在下电化学聚合 2-巯基苯并咪唑（2-MBI），在 Au 电极上制备了聚（2-巯基苯并咪唑）（PMBI）修饰的 MIP。通过原子力显微镜（AFM）、红外光谱（IR）、电化学阻抗谱（EIS）和 CV 对 MIP PMBI 的表面形貌和结构进行了表征。识别的主要驱动力是 MEL 和 PMBI 之间的π-供体-受体相互作用。印迹电极可以成功地避免干扰。此外，从 1.0×10(-8) M 到 5.0×10(-5) M 可以得到线性响应曲线，检测限为 3.0×10(-9) M。该传感器具有较高的灵敏度、较宽的线性范围和较低的检测限等显著优点。该方法有望在未来的食品分析中用于监测非电化学活性物质。
