Regasa Melkamu Biyana, Refera Soreta Tesfaye, Femi Olu Emmanuel, C Ramamurthy Praveen
School of Materials Science and Engineering, Jimma Institute of Technology, Jimma University, Jimma 378, Ethiopia.
Materials Engineering Department, Addis Ababa Institute of Technology, Addis Ababa University, Addis Ababa 1000, Ethiopia.
ACS Omega. 2020 Feb 18;5(8):4090-4099. doi: 10.1021/acsomega.9b03747. eCollection 2020 Mar 3.
Simple, fast, and sensitive molecularly imprinted composite thin-film-based electrochemical sensor developed by using in situ co-electropolymerization of aniline and acrylic acid in the presence of melamine as a template is described here. The prepolymerization complex formation was studied by using Fourier transform infrared (FTIR) spectrophotometry, while the film formation was performed and characterized by cyclic voltammetry, Fourier transform infrared (FTIR), and scanning electron microscopy (SEM). The optimization of important parameters and removal of melamine generated the binding sites in the polymer matrix, which can recognize melamine specifically. Electrochemical measurements were performed to achieve the linear range, the limit of quantification, and limit of detection of 0.1-180, 0.0573, and 0.0172 nM, respectively. The sensitivity of the sensor was attributed to the synergistic effects of amine from aniline and the carboxylic group from acrylic acid to form multiple noncovalent interactions with the template. Melamine-spiked infant formula and raw milk were analyzed by the developed sensor, and the recovery range of 95.87-105.63% with a relative standard deviation of 1.11-2.23% was obtained. The results showed that the developed sensor using the new composite polymer receptor is promising for the online monitoring of melamine in the food industries in the future.
本文描述了一种简单、快速且灵敏的基于分子印迹复合薄膜的电化学传感器，该传感器是通过在三聚氰胺作为模板的存在下，苯胺和丙烯酸的原位共电聚合制备而成。采用傅里叶变换红外（FTIR）分光光度法研究了预聚合配合物的形成，同时通过循环伏安法、傅里叶变换红外（FTIR）和扫描电子显微镜（SEM）对薄膜的形成进行了表征。重要参数的优化和三聚氰胺的去除在聚合物基质中产生了结合位点，该结合位点可以特异性识别三聚氰胺。进行电化学测量以获得线性范围、定量限和检测限，分别为0.1 - 180 nM、0.0573 nM和0.0172 nM。传感器的灵敏度归因于苯胺中的胺基和丙烯酸中的羧基协同作用，与模板形成多种非共价相互作用。用所开发的传感器对添加三聚氰胺的婴儿配方奶粉和原料奶进行了分析，回收率范围为95.87 - 105.63%，相对标准偏差为1.11 - 2.23%。结果表明，所开发的使用新型复合聚合物受体的传感器在未来食品工业中对三聚氰胺的在线监测方面具有广阔前景。
