大肠杆菌O33含甘油2-磷酸的O-特异性多糖的结构与遗传学
Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33.
作者信息
Senchenkova Sof'ya N, Hou Wenqi, Naumenko Olesya I, Geng Peng, Shashkov Alexander S, Perepelov Andrei V, Yang Bin, Knirel Yuriy A
机构信息
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, PR China.
出版信息
Carbohydr Res. 2018 May 2;460:47-50. doi: 10.1016/j.carres.2018.02.008. Epub 2018 Feb 17.
An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O33 followed by gel-permeation chromatography on Sephadex G-50. The polysaccharide was found to contain glycerol 2-phosphate (Gro-2-P), and the following structure of its tetrasaccharide repeat was established by sugar analysis, dephosphorylation, and 1D and 2D H and C NMR spectroscopy: The O33-antigen gene cluster was analyzed and found to be essentially consistent with the O-polysaccharide structure.
通过对大肠杆菌O33脂多糖进行温和酸降解，然后在葡聚糖G-50上进行凝胶渗透色谱法，分离出一种O-特异性多糖。发现该多糖含有甘油2-磷酸（Gro-2-P），并通过糖分析、去磷酸化以及一维和二维氢谱和碳谱核磁共振光谱确定了其四糖重复序列的以下结构：对O33抗原基因簇进行了分析，发现其与O-多糖结构基本一致。
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