Advanced Materials Research Laboratory, Department of Chemistry, Periyar University, Salem 636 011, India.
Mater Sci Eng C Mater Biol Appl. 2018 May 1;86:70-82. doi: 10.1016/j.msec.2018.01.006. Epub 2018 Feb 1.
A new class of bio composite materials for orthopedic applications were prepared by blending polyvinyl alcohol (PVA) with certain amino acids (AA) such as glycine, lysine and phenyl alanine followed by in-situ cross-linking with citric acid (CT). CT cross-linked PVA offers sufficient chemical, thermal, and morphological stability by establishing intermolecular interaction. These biocomposite films were characterized for mechanical and thermal behaviors as well as analyzed through SEM, TGA and AFM studies. Zwitterionic characteristics of AA create cell-adhesive hydrophilic domains that act as potential cell-adhesion sites and facilitate osteointegration. Biocompatibility studies proved that PVA-AA/CT is non-toxic and exhibits good anti-bacterial activity. In-vitro bioactivity test and cell adhesion results predict that presence of AA is advantageous to enhance apatite growth and promote cell-substrate binding through modulating cellular activity of PVA polymer. Hydrophilicity of AA zwitterions significantly facilitates cell-substrate binding and CA cross-linking helps osteoblastic integration of PVA-AA/CT bio composite films. The rational design of microstructure with zwitterionic-hydrophilic domains is a key to enhance cell-substrate interaction of PVA-AAs/CT bio composites.
为了骨科应用，我们通过混合聚乙烯醇（PVA）和某些氨基酸（AA），例如甘氨酸、赖氨酸和苯丙氨酸，然后用柠檬酸（CT）进行原位交联，制备了一类新的生物复合材料。CT 交联的 PVA 通过建立分子间相互作用提供了足够的化学、热和形态稳定性。我们对这些生物复合膜的力学和热性能进行了表征，并通过 SEM、TGA 和 AFM 研究进行了分析。AA 的两性离子特性会产生细胞黏附的亲水结构域，充当潜在的细胞黏附位点，并促进骨整合。生物相容性研究证明，PVA-AA/CT 无毒，具有良好的抗菌活性。体外生物活性测试和细胞黏附结果表明，AA 的存在有利于通过调节 PVA 聚合物的细胞活性来增强磷灰石的生长和促进细胞-基底结合。AA 两性离子的亲水性显著促进了细胞-基底的结合，CA 交联有助于 PVA-AA/CT 生物复合膜的成骨细胞整合。具有两性离子-亲水结构域的微观结构的合理设计是增强 PVA-AAs/CT 生物复合材料细胞-基底相互作用的关键。
