Biomedical Sciences, School of Life Sciences, University of Hull, Hull, UK.
Chem Commun (Camb). 2018 Mar 27;54(26):3227-3230. doi: 10.1039/c8cc00687c.
A selective fluorescent probe for Zn(ii), AQA-F, has been synthesized. AQA-F exhibits a ratiometric shift in emission of up to 80 nm upon binding Zn(ii) ([AQA-F] = 0.1 mM, [Zn(ii)Cl2] = 0-300 μM). An enhancement of quantum yield from Φ = 4.2% to Φ = 35% is also observed. AQA-F has a binding constant, Kd = 15.2 μM with Zn(ii). This probe has been shown to respond to endogenous Zn(ii) levels in vitro in prostate and prostate cancer cell lines. [18F]AQA-F has been synthesized with a radiochemical yield of 8.6% and a radiochemical purity of 97% in 88 minutes. AQA-F shows the potential for a dual modal PET/fluorescence imaging probe for Zn(ii).
一种用于检测 Zn(ii)的选择性荧光探针 AQA-F 已被合成。AQA-F 在与 Zn(ii)结合时，发射光谱发生了高达 80nm 的比率变化（[AQA-F] = 0.1mM，[Zn(ii)Cl2] = 0-300μM）。同时，量子产率也从 Φ = 4.2%提高到了 Φ = 35%。AQA-F 与 Zn(ii)的结合常数为 Kd = 15.2μM。该探针已被证明可以在体外响应前列腺和前列腺癌细胞系中的内源性 Zn(ii)水平。[18F]AQA-F 在 88 分钟内以 8.6%的放射化学产率和 97%的放射化学纯度合成。AQA-F 有望成为一种用于 Zn(ii)的双模态 PET/荧光成像探针。
