School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
J Infect Public Health. 2015 Mar-Apr;8(2):202-6. doi: 10.1016/j.jiph.2014.09.002. Epub 2014 Oct 29.
The aim of this study was to determine the prevalence and characteristics of Staphylococcus aureus isolates from the patients, staff, air and environments of an ICU in a hospital in Tehran.
During this study, 37 S. aureus isolates were collected and analyzed via the spa typing method.
Of the 37 S. aureus isolates, 35 (94%) were methicillin resistant (MRSA), 28 (76%) were identified as SCCmec types III or IIIA, four (10%) were identified as SCCmec types I or IA and three (8%) were identified a SCCmec type IV. All of the MRSA isolates were resistant to oxacillin and contained mecA. The isolates were all spa typed and found to comprise 11 spa types, including t7688, t7689, and t7789, which have not previously been reported. The spa type t7688 was isolated from the hands of two ICU personnel. The spa type t7689 was observed among five isolates from the air and the environment. The spa type t7789 was observed among three isolates from the patients, ventilators and the air. The majority of the isolates (43%) belonged to spa types t030 and t037.
Our results revealed that MRSA strains that were isolated from the air, the environment of the ICU and the patients who were colonized or infected with MRSA often exhibited the same spa and SCCmec types. These results also reveal that the isolates from the patients and environment were usually indistinguishable.
本研究旨在确定德黑兰一家医院 ICU 患者、工作人员、空气和环境中分离的金黄色葡萄球菌的流行率和特征。
在这项研究中，通过 spa 分型方法收集和分析了 37 株金黄色葡萄球菌分离株。
37 株金黄色葡萄球菌分离株中，35 株（94%）为耐甲氧西林金黄色葡萄球菌（MRSA），28 株（76%）鉴定为 SCCmec 型 III 或 IIIA，4 株（10%）鉴定为 SCCmec 型 I 或 IA，3 株（8%）鉴定为 SCCmec 型 IV。所有 MRSA 分离株均对苯唑西林耐药，且均含有 mecA。所有分离株均进行 spa 分型，共发现 11 种 spa 型，包括 t7688、t7689 和 t7789，此前尚未报道过。spa 型 t7688 从两名 ICU 工作人员的手上分离得到。spa 型 t7689 见于空气和环境中 5 株分离株。spa 型 t7789 见于 3 株患者、呼吸机和空气中的分离株。大多数分离株（43%）属于 spa 型 t030 和 t037。
我们的结果表明，从空气、ICU 环境和定植或感染 MRSA 的患者中分离出的 MRSA 菌株通常具有相同的 spa 和 SCCmec 型。这些结果还表明，患者和环境中的分离株通常无法区分。
