Tayel Safaa I, Soliman Shimaa E, Elsayed Hanan M
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Egypt.
Steroids. 2018 Jun;134:37-42. doi: 10.1016/j.steroids.2018.03.003. Epub 2018 Mar 10.
Increasing prevalence of neonatal sepsis in recent years catch attention to early prevention and management. Vitamin D receptor (VDR) polymorphism can modulate VDR expression level that greatly influences immunity and susceptibility to microbial infections. We aimed to investigate the association of VDR polymorphism at FokI, rs2228570 T/C, and TaqI, rs731236 C/T gene with serum 25-hydroxyvitamin D level and risk of neonatal sepsis.
This work carried on 160 subjects classified into 80 cases (40 mothers and their 40 septic neonates) and 80 healthy controls (40 volunteer mothers and their 40 healthy neonates). Genotyping of VDR polymorphisms were assayed by real-time PCR and serum 25-hydroxyvitamin D level and hs-CRP were measured by ELISA.
Vitamin D deficiency was observed in mothers of cases compared with healthy ones (p = <0.001) and in septic neonates versus healthy ones (p = <0.001). Septic neonates had much higher VDR FokI TT genotype (p = 0.014) and T allele (p = 0.003) versus healthy ones. TT genotype and T allele could increase the risk of sepsis with OR 95% CI [4.804 (1.4-16.4)] and [2.786 (1.4-5.7)] respectively while VDR TaqI showed no association with sepsis. There was a strong LD between FokI and TaqI in sepsis cases. In sepsis, T/T genotype at FokI had significantly lower vitamin D (p = <0.001).
Vitamin D deficiency in mothers/neonates is a risk factor for neonatal sepsis. VDR FokI T allele had lower 25-hydroxyvitamin D level that may predispose to sepsis hazards.
近年来新生儿败血症患病率的上升引起了人们对早期预防和管理的关注。维生素D受体（VDR）多态性可调节VDR表达水平，这对免疫力和微生物感染易感性有很大影响。我们旨在研究FokI位点（rs2228570 T/C）、TaqI位点（rs731236 C/T）VDR多态性与血清25-羟基维生素D水平及新生儿败血症风险的关联。
本研究纳入160名受试者，分为80例（40名母亲及其40名患败血症的新生儿）和80名健康对照（40名志愿者母亲及其40名健康新生儿）。采用实时PCR法检测VDR多态性的基因分型，采用ELISA法检测血清25-羟基维生素D水平和高敏C反应蛋白（hs-CRP）。
与健康母亲相比，病例组母亲中观察到维生素D缺乏（p＜0.001）；与健康新生儿相比，患败血症的新生儿中也观察到维生素D缺乏（p＜0.001）。与健康新生儿相比，患败血症的新生儿具有更高的VDR FokI TT基因型（p = 0.014）和T等位基因（p = 0.003）。TT基因型和T等位基因可分别增加败血症风险，其比值比（OR）及95%置信区间（CI）分别为[4.804（1.4 - 16.4）]和[2.786（1.4 - 5.7）]；而VDR TaqI与败血症无关联。在败血症病例中，FokI和TaqI之间存在强连锁不平衡（LD）。在败血症中，FokI的T/T基因型维生素D水平显著较低（p＜0.001）。
母亲/新生儿维生素D缺乏是新生儿败血症的危险因素。VDR FokI T等位基因25-羟基维生素D水平较低可能易引发败血症风险。
