Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur 176 061, Himachal Pradesh, India.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Post Box No. 06, Palampur 176 061, Himachal Pradesh, India.
Genomics. 2019 May;111(3):492-499. doi: 10.1016/j.ygeno.2018.03.008. Epub 2018 Mar 9.
Pseudomonas frederiksbergensis ERDD5:01 is a psychrotrophic bacteria isolated from the glacial stream flowing from East Rathong glacier in Sikkim Himalaya. The strain showed survivability at high altitude stress conditions like freezing, frequent freeze-thaw cycles, and UV-C radiations. The complete genome of 5,746,824 bp circular chromosome and a plasmid of 371,027 bp was sequenced to understand the genetic basis of its survival strategy. Multiple copies of cold-associated genes encoding cold active chaperons, general stress response, osmotic stress, oxidative stress, membrane/cell wall alteration, carbon storage/starvation and, DNA repair mechanisms supported its survivability at extreme cold and radiations corroborating with the bacterial physiological findings. The molecular cold adaptation analysis in comparison with the genome of 15 mesophilic Pseudomonas species revealed functional insight into the strategies of cold adaptation. The genomic data also revealed the presence of industrially important enzymes.
弗雷德里克斯堡假单胞菌 ERDD5:01 是一种嗜冷细菌，从锡金喜马拉雅山脉东拉特洪冰川流出的冰川溪流中分离出来。该菌株在高海拔应激条件下如冷冻、频繁的冻融循环和 UV-C 辐射下具有生存能力。对 5746824bp 环状染色体和 371027bp 的质粒进行了全基因组测序，以了解其生存策略的遗传基础。大量的冷相关基因编码冷活性伴侣、一般应激反应、渗透应激、氧化应激、膜/细胞壁改变、碳储存/饥饿和 DNA 修复机制，支持其在极端寒冷和辐射下的生存能力，与细菌的生理发现相吻合。与 15 种嗜温假单胞菌的基因组进行的分子冷适应分析揭示了对冷适应策略的功能见解。基因组数据还显示了存在工业上重要的酶。
