Department of Thoracic Surgery, Second Xiangya Hospital of Central South University, Hunan, China.
Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.
Thorac Cancer. 2018 May;9(5):589-597. doi: 10.1111/1759-7714.12623. Epub 2018 Mar 13.
For many years, lung cancer has been the most common and deadly cancer worldwide. Early diagnosis of non-small cell lung cancer (NSCLC) in particular is very difficult because the symptoms are often ignored. The five-year survival rate is very low despite great improvements to therapy. Thus, there is an urgent need to identify prognostic biomarkers and target molecules for the clinical diagnosis and individualized treatment of NSCLC.
We performed quantitative real-time PCR to determine the expression levels of the long non-coding RNA (lncRNA) linc01433 in NSCLC and normal matched lung tissue. Subsequently, we established cell lines with overexpression or knockdown of linc01433 to evaluate the effects on proliferation and metastasis in vitro. Epithelial-to-mesenchymal transition was examined using Western blot.
Linc01433 was significantly overexpressed in NSCLC tissues compared to normal lung tissues. In addition, linc01433 levels were associated with smoking history. Linc01433 overexpression in lung cancer cells increased proliferation, migration, and invasion abilities, as well as epithelial-to-mesenchymal transition.
Linc01433 is a cancer-related lncRNA that may have an oncogene-like effect in NSCLC.
多年来，肺癌一直是全球最常见和最致命的癌症。特别是非小细胞肺癌（NSCLC）的早期诊断非常困难，因为这些症状经常被忽视。尽管治疗方法有了很大的改进，五年生存率仍然很低。因此，迫切需要确定 NSCLC 的预后生物标志物和靶向分子，用于临床诊断和个体化治疗。
我们采用实时定量 PCR 法检测 NSCLC 及配对正常肺组织中长链非编码 RNA（lncRNA）linc01433 的表达水平。随后，我们通过过表达或敲低 linc01433 建立细胞系，以评估其对体外增殖和转移的影响。采用 Western blot 法检测上皮间质转化。
与正常肺组织相比，linc01433 在 NSCLC 组织中明显过表达。此外，linc01433 水平与吸烟史有关。肺癌细胞中 linc01433 的过表达增加了增殖、迁移和侵袭能力以及上皮间质转化。
Linc01433 是一种与癌症相关的 lncRNA，在 NSCLC 中可能具有癌基因样作用。
