Department of Human and Animal Cell Lines, Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig, Germany.
Biol Chem. 2018 May 24;399(6):511-523. doi: 10.1515/hsz-2017-0321.
Characteristic components of Hodgkin lymphoma (HL) tissue are the mono- or multinucleated Hodgkin-Reed-Sternberg (HRS) cells. Given the challenges of isolating these rare malignant cells and the difficulty in culturing cells from patients, many investigators have tried to establish cell lines in efforts to develop cellular tools for in vitro studies. A limited number of HL cell lines exist and have provided valuable insights into HL pathobiology. A literature survey indicated that 35 cell lines derived from HL patients have been published. To determine whether all these alleged HL cell lines hold up to scrutiny, we examined the available data and also put some of these cell lines to the test of hierarchical clustering, providing additional information regarding assignment to cell line type and tissue derivation. Hierarchical clustering separated the bona fide (classical) HL cell lines completely from cell lines derived from other lymphoma categories and proved conclusively that HL cell lines represent a distinct entity, irrespective of the cellular origin of the HRS cells. We conclude by pointing out the need for an intensified search for new cell culture avenues in order to develop a new generation of informative HL cell lines covering more widely the spectrum of HL stages and subtypes.
霍奇金淋巴瘤（HL）组织的特征性成分是单核或多核的霍奇金-里德-斯特恩伯格（HRS）细胞。鉴于分离这些罕见的恶性细胞具有挑战性，且从患者中培养细胞较为困难，许多研究人员尝试建立细胞系，以开发用于体外研究的细胞工具。目前已经建立了少量的 HL 细胞系，这些细胞系为 HL 病理生物学提供了有价值的见解。文献调查显示，已经发表了 35 种源自 HL 患者的细胞系。为了确定所有这些所谓的 HL 细胞系是否经得起仔细审查，我们检查了可用的数据，并对其中一些细胞系进行了层次聚类分析，提供了有关细胞系类型和组织来源分配的更多信息。层次聚类将真正的（经典）HL 细胞系与源自其他淋巴瘤类别的细胞系完全分开，这确凿地证明了 HL 细胞系是一个独特的实体，而与 HRS 细胞的细胞来源无关。最后，我们指出需要加强寻找新的细胞培养途径的研究，以开发新一代具有信息性的 HL 细胞系，更广泛地涵盖 HL 阶段和亚型的范围。
