细胞 hnRNP A1 与猪流行性腹泻病毒核衣壳蛋白相互作用并抑制病毒复制。

Cellular hnRNP A1 Interacts with Nucleocapsid Protein of Porcine Epidemic Diarrhea Virus and Impairs Viral Replication.

机构信息

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Viruses. 2018 Mar 13;10(3):127. doi: 10.3390/v10030127.

The nucleocapsid (N) protein is a major structural component of porcine epidemic diarrhea virus (PEDV), which is predicted to be a multifunctional protein in viral replication. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a cellular protein participating in the splicing of pre-mRNA in the nucleus and translation regulation in the cytoplasm. According to our previous proteomic study about PEDV infection in vivo, hnRNP A1 was thought to be a cellular factor influencing PEDV replication. In this report, PEDV N protein was discovered to colocalize with cellular hnRNP A1 in perinuclear region of PEDV infected cells. Co-immunoprecipitation (CO-IP) results clearly demonstrated that PEDV N protein could bind to human hnRNP A1. Replication of PEDV was inhibited by silencing the expression of hnRNP A1 in CCL-81 cells, suggesting the positive effect of hnRNP A1 on PEDV infection.

核衣壳（N）蛋白是猪流行性腹泻病毒（PEDV）的主要结构成分，据预测是病毒复制中的一种多功能蛋白。异质核核糖核蛋白 A1（hnRNP A1）是一种参与核内前体 mRNA 剪接和细胞质翻译调节的细胞蛋白。根据我们之前关于 PEDV 体内感染的蛋白质组学研究，hnRNP A1 被认为是影响 PEDV 复制的细胞因子。在本报告中，发现 PEDV N 蛋白与感染细胞的核周区的细胞 hnRNP A1 共定位。免疫共沉淀（CO-IP）结果清楚地表明，PEDV N 蛋白可以与人 hnRNP A1 结合。在 CCL-81 细胞中沉默 hnRNP A1 的表达抑制了 PEDV 的复制，这表明 hnRNP A1 对 PEDV 感染具有正向作用。