Key Laboratory of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2018 Mar 10;19(3):782. doi: 10.3390/ijms19030782.
Leaf senescence is an important agronomic trait that affects both crop yield and quality. In this study, we characterized a premature leaf senescence mutant of wheat ( L.) obtained by ethylmethane sulfonate (EMS) mutagenesis, named . Genetic analysis showed that the leaf senescence phenotype of is controlled by a single recessive nuclear gene. We compared the transcriptome of wheat leaves between the wild type (WT) and the mutant at four time points. Differentially expressed gene (DEG) analysis revealed many genes that were closely related to senescence genes. Gene Ontology (GO) enrichment analysis suggested that transcription factors and protein transport genes might function in the beginning of leaf senescence, while genes that were associated with chlorophyll and carbon metabolism might function in the later stage. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the genes that are involved in plant hormone signal transduction were significantly enriched. Through expression pattern clustering of DEGs, we identified 1012 genes that were induced during senescence, and we found that the family and zinc finger transcription factors might be more important than other transcription factors in the early stage of leaf senescence. These results will not only support further gene cloning and functional analysis of , but also facilitate the study of leaf senescence in wheat.
叶片衰老是影响作物产量和品质的重要农艺性状。本研究通过甲基磺酸乙酯（EMS）诱变，获得了一个小麦（L.）的过早叶片衰老突变体，命名为 。遗传分析表明， 的叶片衰老表型由一个单隐性核基因控制。我们比较了野生型（WT）和 突变体在四个时间点的小麦叶片转录组。差异表达基因（DEG）分析表明，许多基因与衰老基因密切相关。基因本体论（GO）富集分析表明，转录因子和蛋白质转运基因可能在叶片衰老的早期起作用，而与叶绿素和碳代谢相关的基因可能在后期起作用。京都基因与基因组百科全书（KEGG）途径分析表明，参与植物激素信号转导的基因显著富集。通过 DEG 的表达模式聚类，我们鉴定了 1012 个在衰老过程中诱导的基因，我们发现 家族和锌指转录因子在叶片衰老的早期可能比其他转录因子更为重要。这些结果不仅将支持对 的进一步基因克隆和功能分析，也将有助于小麦叶片衰老的研究。
