Beirão Breno C B, Ingberman Max, Fávaro Celso, Mesa Dany, Bittencourt Letícia C, Fascina Vitor B, Caron Luiz Felipe
a Imunova Análises Biológicas , Setor de Ciências Biológicas UFPR , Curitiba , Brazil.
b Setor de Ciências Biológicas UFPR, Universidade Federal do Paraná , Curitiba , Brazil.
Avian Pathol. 2018 Jun;47(3):325-333. doi: 10.1080/03079457.2018.1450487. Epub 2018 Apr 6.
Probiotics and immunization are being widely adopted by the poultry industry with the goal of controlling Salmonella enterica. However, the interaction between these two management protocols has been sparsely studied. The present study aimed to understand the role of an Enterococcus faecium probiotic in the production of salmonella-specific IgA in layers immunized with a live vaccine. Four groups were used: "Control" (no vaccine or probiotic); "Probiotic" (which received an E. faecium product); "Vaccine" (immunized with two doses of a live attenuated S. Enteritidis vaccine); and "Vaccine + probiotic". Faecal salmonella-specific IgA was analysed 7 and 20 days post-vaccination (dpv) boost. At 7 dpv, the "Vaccine" and "Vaccine + probiotic" groups had similar IgA levels. However, at 20 dpv, IgA levels were two times higher in the "Vaccine + probiotic" group compared to the "Vaccine" group. To understand the role of the intestinal microbiota in this finding, bacterial diversity in faeces was analysed by 16S rRNA gene sequencing. The improvement in IgA production in probiotic-treated birds was accompanied by marked changes in the faecal microbiome. Some of the main differences between the "Vaccine" and "Vaccine + probiotic" groups included reduction of Escherichia-Shigella and increases in Blautia, Anaerotruncus and Lactobacillus in the latter group. Although no direct causal link can be established from this study design, it is possible that the E. faecium probiotic induces improved antibody production following vaccination via modulation of the intestinal microbiota.
益生菌和免疫接种正被家禽业广泛采用，目的是控制肠炎沙门氏菌。然而，这两种管理方案之间的相互作用鲜有研究。本研究旨在了解粪肠球菌益生菌在用活疫苗免疫的蛋鸡中产生沙门氏菌特异性IgA的作用。实验使用了四组：“对照组”（未接种疫苗或使用益生菌）；“益生菌组”（接受粪肠球菌产品）；“疫苗组”（用两剂减毒活肠炎沙门氏菌疫苗免疫）；以及“疫苗+益生菌组”。在加强免疫接种后7天和20天（dpv）分析粪便中沙门氏菌特异性IgA。在7 dpv时，“疫苗组”和“疫苗+益生菌组”的IgA水平相似。然而，在20 dpv时，“疫苗+益生菌组”的IgA水平比“疫苗组”高两倍。为了解肠道微生物群在此发现中的作用，通过16S rRNA基因测序分析粪便中的细菌多样性。益生菌处理的鸡中IgA产生的改善伴随着粪便微生物群的显著变化。“疫苗组”和“疫苗+益生菌组”之间的一些主要差异包括，后者中埃希氏菌-志贺氏菌减少，而布劳特氏菌、厌氧短杆菌和乳酸杆菌增加。尽管从本研究设计中无法建立直接的因果联系，但粪肠球菌益生菌有可能通过调节肠道微生物群诱导疫苗接种后抗体产生的改善。
