Revajová Viera, Benková Terézia, Karaffová Viera, Levkut Martin, Selecká Emília, Dvorožňáková Emília, Ševčíková Zuzana, Herich Róbert, Levkut Mikuláš
Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, 041 81 Košice, Slovakia.
Institute of Parasitology, Slovak Academy of Sciences, 040 01 Košice, Slovakia.
Life (Basel). 2022 Jan 28;12(2):201. doi: 10.3390/life12020201.
Immune response of day-old chicks infected with Enteritidis PT4 and preventive administration of AL41 were studied using hematology and flow cytometry of immunocompetent cells in blood, cecum, bursa and spleen for 11 days, and included 220 animals divided into four groups (n = 55). AL41 was administered for 7 days to EF and EFSE groups and on day 4 SE and EFSE groups were infected with Enteritidis. Values of monocytes at 4 dpi significantly increased in EFSE and lymphocytes at 7 dpi in EF groups. Blood CD3, CD4, CD8 and IgM lymphocytes improved in EF and EFSE groups and IgA in EF group at 4 dpi. Phagocytic activity of probiotic groups was improved in both samples. Cecal IEL and LPL lymphocytes showed at 7 dpi stimulation of CD3, CD4 and CD8 subpopulations in probiotic groups, especially in EFSE group, IgA IEL and IgA with IgM LPL in EF groups. Bursa Fabricii at 7 dpi presented overstimulation of IgG subpopulation in SE group, spleen CD3 and CD8 in EF and EFSE groups. AL41 revealed the protective effect and positive influence on the local and systemic immune response in Enteritidis PT4 infected chickens.
利用血液、盲肠、法氏囊和脾脏中免疫活性细胞的血液学和流式细胞术，对感染肠炎沙门氏菌PT4的1日龄雏鸡的免疫反应以及AL41的预防性给药进行了为期11天的研究，研究包括220只动物，分为四组（n = 55）。EF组和EFSE组给予AL41 7天，SE组和EFSE组在第4天感染肠炎沙门氏菌。EFSE组在感染后第4天单核细胞值显著增加，EF组在感染后第7天淋巴细胞值显著增加。EF组和EFSE组血液中的CD3、CD4、CD8和IgM淋巴细胞在感染后第4天有所改善，EF组的IgA在感染后第4天有所改善。益生菌组的吞噬活性在两个样本中均有所提高。盲肠IEL和LPL淋巴细胞在感染后第7天显示益生菌组中CD3、CD4和CD8亚群受到刺激，尤其是EFSE组，EF组中IgA IEL和IgA与IgM LPL。法氏囊在感染后第7天显示SE组中IgG亚群过度刺激，EF组和EFSE组脾脏中的CD3和CD8。AL41对感染肠炎沙门氏菌PT4的鸡的局部和全身免疫反应具有保护作用和积极影响。
