Influence of Immune Parameters after AL41 Administration and Infection in Chickens.

Immune response of day-old chicks infected with Enteritidis PT4 and preventive administration of AL41 were studied using hematology and flow cytometry of immunocompetent cells in blood, cecum, bursa and spleen for 11 days, and included 220 animals divided into four groups (n = 55). AL41 was administered for 7 days to EF and EFSE groups and on day 4 SE and EFSE groups were infected with Enteritidis. Values of monocytes at 4 dpi significantly increased in EFSE and lymphocytes at 7 dpi in EF groups. Blood CD3, CD4, CD8 and IgM lymphocytes improved in EF and EFSE groups and IgA in EF group at 4 dpi. Phagocytic activity of probiotic groups was improved in both samples. Cecal IEL and LPL lymphocytes showed at 7 dpi stimulation of CD3, CD4 and CD8 subpopulations in probiotic groups, especially in EFSE group, IgA IEL and IgA with IgM LPL in EF groups. Bursa Fabricii at 7 dpi presented overstimulation of IgG subpopulation in SE group, spleen CD3 and CD8 in EF and EFSE groups. AL41 revealed the protective effect and positive influence on the local and systemic immune response in Enteritidis PT4 infected chickens.