Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center-Campbell Clinic.
Veterans Affairs Medical Center, Memphis, TN, USA.
Int J Nanomedicine. 2018 Mar 1;13:1215-1224. doi: 10.2147/IJN.S149375. eCollection 2018.
Early stage osteoarthritis (OA) is clinically asymptomatic due to the avascular and the aneural nature of the cartilage tissue. Nevertheless, early detection of cartilage tissue is critical in order to impede the progression of OA. Hence, in order to develop effective preventive therapy for OA, diagnosis in the early stages is necessary.
To achieve this goal, we have developed targeted, fluorescent nanosomes conjugated with monoclonal anti-type II collagen antibodies (MabCII) for diagnosis of early OA. The MabCII-coated nanosomes (targeted-nanosomes) bind to the damaged cartilage explants in vitro and in vivo in an OA mouse model that mimics early stage OA. The OA mouse model was induced by destabilization of the medial meniscus (DMM) in 9-10 weeks old C57Bl/6 mice.
The targeted-nanosomes enhanced the binding specificity to the cartilage tissue according to the severity of damage.
We show that MabCII-nanosomes can precisely detect early stage OA in the DMM mouse model. Thus, MabCII-nanosomes have the potential to be used as a non-invasive method for diagnosing the early osteoarthritic lesions.
由于软骨组织的血管和神经缺乏，早期骨关节炎（OA）在临床上是无症状的。然而，早期发现软骨组织对于阻止 OA 的进展至关重要。因此，为了开发有效的 OA 预防疗法，有必要在早期进行诊断。
为了实现这一目标，我们开发了靶向、荧光纳米囊，其与单克隆抗 II 型胶原抗体（MabCII）偶联，用于诊断早期 OA。MabCII 包被的纳米囊（靶向纳米囊）在体外和体内与模拟早期 OA 的 OA 小鼠模型中的受损软骨外植体结合。OA 小鼠模型通过在 9-10 周龄 C57Bl/6 小鼠中破坏内侧半月板（DMM）来诱导。
根据损伤的严重程度，靶向纳米囊增强了与软骨组织的结合特异性。
我们表明，MabCII-纳米囊可以精确检测 DMM 小鼠模型中的早期 OA。因此，MabCII-纳米囊有可能成为诊断早期骨关节炎病变的非侵入性方法。
