Park Miri, Song Jaeho, Nam Gi Gyun, Kim Suhyun, Joung Yochan, Cho Jang-Cheon
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2018 May;68(5):1565-1570. doi: 10.1099/ijsem.0.002712. Epub 2018 Mar 14.
A bacterial strain, designated IMCC25901, was isolated from a freshwater lake, Soyang, in the Republic of Korea. The strain was Gram-stain-negative, aerobic, non-motile, orange-coloured and short rod-shaped. The 16S rRNA gene sequence analysis showed that strain IMCC25901 was most closely related to Flavobacterium yonginense HMD1001 (97.0 %) and formed a robust phylogenetic clade with other species of the genus Flavobacterium. Growth of strain IMCC25901 was observed at 10-30 °C (optimum, 20 °C), pH 6-8 (optimum, pH 7) and 0-1.0 % NaCl (optimum, 0 %). The DNA G+C content of strain IMCC25901 was 34.2 mol%. The major fatty acid constituents of the strain were anteiso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C15 : 0. Cells of strain IMCC25901 contained phosphatidylethanolamine, an unidentified phospholipid, two unidentified aminolipids and two unidentified lipids. The isoprenoid quinone detected in the strain was MK-6. On the basis of the taxonomic data obtained in this study, it was concluded that strain IMCC25901 represented a novel species in the genus Flavobacterium, for which the name Flavobacterium lacicola sp. nov. is proposed. The type strain of Flavobacterium lacicola is IMCC25901 (=KCTC 52571=NBRC 112883).
从韩国昭阳淡水湖分离出一株细菌，命名为IMCC25901。该菌株革兰氏染色阴性，好氧，无运动性，橙色，短杆状。16S rRNA基因序列分析表明，菌株IMCC25901与龙仁黄杆菌HMD1001关系最为密切（相似度为97.0%），并与黄杆菌属的其他物种形成了一个稳定的系统发育分支。在10-30°C（最适温度为20°C）、pH 6-8（最适pH为7）和0-1.0% NaCl（最适盐浓度为0%）条件下观察到菌株IMCC25901的生长。菌株IMCC25901的DNA G+C含量为34.2 mol%。该菌株的主要脂肪酸成分是anteiso-C15:0、总和特征3（C16:1ω6c和/或C16:1ω7c）和iso-C15:0。菌株IMCC25901的细胞含有磷脂酰乙醇胺、一种未鉴定的磷脂、两种未鉴定的氨基脂质和两种未鉴定的脂质。该菌株中检测到的类异戊二烯醌为MK-6。根据本研究获得的分类学数据，得出结论：菌株IMCC25901代表黄杆菌属中的一个新物种，为此提出了新名称黄杆菌湖生种（Flavobacterium lacicola sp. nov.）。黄杆菌湖生种（Flavobacterium lacicola）的模式菌株是IMCC25901（=KCTC 52571=NBRC 112883）。
