Nam Gi Gyun, Joung Yochan, Park Miri, Kim Suhyun, Jeon Hyoung Tae, Cho Jang-Cheon
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2440-2445. doi: 10.1099/ijsem.0.001987. Epub 2017 Jul 26.
A bacterial strain, designated IMCC26223T, was isolated from an oligotrophic freshwater lake, Lake Soyang, Korea. Cells of strain IMCC26223T were Gram-staining negative, strictly aerobic, non-motile and short-rod-shaped. Growth occurred at pH 6-8 (optimum, pH 7.0), at 4-25 °C (optimum, 15 °C) and with 0-0.5 % (w/v) NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain IMCC26223T was identified as a member of the genus Flavobacterium and most closely related to Flavobacterium fluvii H7T (97.6 %), Flavobacterium segetis AT1048T (97.5 %) and Flavobacterium weaverense AT1042T (97.2 %). DNA-DNA relatedness between strain IMCC26223T and F. fluvii H7T was 41.5-51.7 % in the reciprocal hybridization. Strain IMCC26223T contained MK-6 as the major respiratory quinone. The major cellular fatty acids consisted of C16 : 0, iso-C15 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), and the polar lipids were phosphatidylethanolamine, an unidentified aminolipid and two unidentified lipids. The DNA G+C content of strain IMCC26223T was 34.5 mol%. On the basis of 16S rRNA gene phylogeny and phenotypic characterization, strain IMCC26223T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium soyangense sp. nov., is proposed. The type strain is IMCC26223T (=KCTC 52245T=JCM 31384T).
从韩国昭阳湖的贫营养淡水湖中分离出一株细菌，命名为IMCC26223T。IMCC26223T菌株的细胞革兰氏染色阴性，严格需氧，不运动，呈短杆状。在pH 6 - 8（最适pH 7.0）、4 - 25 °C（最适15 °C）和0 - 0.5 %（w/v）NaCl（最适0 %）条件下生长。基于16S rRNA基因序列的系统发育分析表明，IMCC26223T菌株被鉴定为黄杆菌属的一个成员，与河流黄杆菌H7T（97.6 %）、田野黄杆菌AT1048T（97.5 %）和韦弗黄杆菌AT1042T（97.2 %）关系最为密切。在反向杂交中，IMCC26223T菌株与河流黄杆菌H7T之间的DNA - DNA相关性为41.5 - 51.7 %。IMCC26223T菌株以MK - 6作为主要呼吸醌。主要细胞脂肪酸由C16 : 0、异C15 : 0、anteiso - C15 : 0和总和特征3（C16 : 1ω6c和/或C16 : 1ω7c）组成，极性脂质为磷脂酰乙醇胺、一种未鉴定的氨基脂质和两种未鉴定的脂质。IMCC26223T菌株的DNA G + C含量为34.5 mol%。基于16S rRNA基因系统发育和表型特征，IMCC26223T菌株代表黄杆菌属的一个新物种，为此提出新名称昭阳黄杆菌（Flavobacterium soyangense sp. nov.）。模式菌株为IMCC26223T（=KCTC 52245T=JCM 31384T）。
