University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil
University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil.
J Clin Microbiol. 2018 Apr 25;56(5). doi: 10.1128/JCM.01732-17. Print 2018 May.
spp. are bacteria of worldwide distribution that cause asymptomatic to fatal infections in animals and humans. The most common zoonotic species is , for which cats are the major natural reservoir host. To better understand sp. diagnostic limitations, we determined the frequency of bloodstream infection in 112 cats by comparing and combining the results of multiple conventional and nested PCRs from blood and liquid culture samples. Using liquid culture conventional PCR, sp. DNA was amplified from 27.7% of samples (31/112) compared to 90.2% of samples (101/112) by combining nested PCR from blood and liquid culture, indicating that PCR testing of more than one type of sample provides better sensitivity than a standalone PCR and that bloodstream infection is very frequent among cats in southeastern Brazil. This study reinforces the need for multistep testing for sp. infection to prevent false-negative diagnostic results, even in reservoir hosts such as cats that typically maintain higher bacteremia levels.
spp. 是分布于全球的细菌，可引起动物和人类无症状至致命的感染。最常见的人畜共患病种是 ，猫是其主要的天然储存宿主。为了更好地了解 sp. 的诊断局限性，我们通过比较和结合来自血液和液体培养样本的多种常规和嵌套 PCR 的结果，确定了 112 只猫中血流感染的频率。与仅使用血液常规 PCR 相比，使用液体培养常规 PCR 从 27.7%（31/112）的样本中扩增出了 sp. DNA，而通过结合血液和液体培养的嵌套 PCR 则从 90.2%（101/112）的样本中扩增出了 sp. DNA，这表明对多种类型的样本进行 PCR 检测比单独进行 PCR 检测具有更高的敏感性，并且血流感染在巴西东南部的猫中非常普遍。本研究强调了需要对 sp. 感染进行多步骤检测，以防止假阴性的诊断结果，即使在猫等通常保持较高菌血症水平的储存宿主中也是如此。
