Applied Research in Dermatology and Bartonella Infection Laboratory, University of Campinas, UNICAMP, Campinas, São Paulo, Brazil.
Division of Dermatology, Department of Medicine, UNICAMP, Campinas, São Paulo, Brazil.
PLoS Negl Trop Dis. 2022 Jul 18;16(7):e0010603. doi: 10.1371/journal.pntd.0010603. eCollection 2022 Jul.
This study aimed to assess the prevalence of Bartonella sp.-DNA detection in blood and skin samples from patients with non-viral end-stage liver disease awaiting liver transplantation.
METHODOLOGY/PRINCIPAL FINDINGS: Blood samples and healthy skin fragments from 50 patients were tested using microbiological and molecular methods. Fifteen patients had cryptogenic hepatitis (CH) and 35 had alcoholic, drug-induced or autoimmune liver disease. DNA was extracted from whole blood and liquid culture samples, isolates, and skin fragments. Thirteen of the 50 patients (26%) had Bartonella henselae DNA detection in their blood (9/50) and/or skin (5/50) samples. Colonies were isolated in 3/50 (6%) and infection was detected in 7/50 (14%) of the 50 patients. B. henselae-DNA detection was more prevalent in patients with CH than in other patients (p = 0.040). Of 39 patients followed-up for at least two years, a higher mortality rate was observed among patients with CH infected with B. henselae (p = 0.039).
CONCLUSIONS/SIGNIFICANCE: Further studies assessing the role of B. henselae infection in the pathogenesis of hepatitis patients must be urgently conducted.
本研究旨在评估在等待肝移植的非病毒性终末期肝病患者的血液和皮肤样本中，巴尔通体属-DNA 的检测 prevalence。
方法/主要发现：采用微生物学和分子方法检测了 50 例患者的 blood 样本和健康 skin 片段。15 例患者患有隐源性肝炎（CH），35 例患者患有酒精性、药物性或自身免疫性 liver 疾病。从 whole blood 和 liquid culture 样本、isolate 和 skin 片段中提取 DNA。在 50 例患者中的 13 例（26%）的 blood（9/50）和/或 skin（5/50）样本中检测到亨氏巴尔通体 DNA。在 50 例患者中的 3 例（6%）中分离出菌落，在 7 例（14%）中检测到感染。在 CH 患者中，B. henselae-DNA 的检测 prevalence 高于其他患者（p = 0.040）。在至少随访两年的 39 例患者中，感染 B. henselae 的 CH 患者的死亡率更高（p = 0.039）。
结论/意义：必须 urgently 开展进一步的研究，以评估 B. henselae 感染在肝炎患者发病机制中的作用。
