Suppr超能文献

[组织切片中核体积、DNA含量及核形态的表述(作者译)]

[The statement of nuclear volume, DNA content and nuclear form in tissue sections (author's transl)].

作者信息

Anton H J, Thelen R

出版信息

Microsc Acta Suppl. 1979(3):161-9.

PMID:295407
Abstract

15 days after amputation of the forelimb of young postmethamorphic Triturus vulgaris DNA-content and volume of the nuclei of the mesodermal blastema cells have been investigated in longitudinal sections of 2 microns in thickness by Feulgen-photometric and electronic determination of area. Discrimination of the DNA-synthesis phase was carried out using microautoradiography after "in vivo" labeling with tritiated thymidine. Volume and DNA-content of the nuclear sections have been found to be positive correlated. The DNA-synthesising part of the population shows characteristic differences to the mean value of volume and DNA-content of the remaining population. The factor circumference 2/area is suitable to characterize the differentiation degree of cells.

摘要

在幼年变态后的普通欧螈前肢截肢15天后,通过福尔根光度法和电子面积测定法,对厚度为2微米的纵切片中中胚层芽基细胞的细胞核DNA含量和体积进行了研究。在用氚标记的胸腺嘧啶核苷进行“体内”标记后,使用微放射自显影术对DNA合成期进行了鉴别。已发现核切片的体积与DNA含量呈正相关。群体中进行DNA合成的部分与其余群体的体积和DNA含量平均值表现出特征性差异。“周长2/面积”这一因子适用于表征细胞的分化程度。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验