Anton H J, Thelen R
Microsc Acta Suppl. 1979(3):161-9.
15 days after amputation of the forelimb of young postmethamorphic Triturus vulgaris DNA-content and volume of the nuclei of the mesodermal blastema cells have been investigated in longitudinal sections of 2 microns in thickness by Feulgen-photometric and electronic determination of area. Discrimination of the DNA-synthesis phase was carried out using microautoradiography after "in vivo" labeling with tritiated thymidine. Volume and DNA-content of the nuclear sections have been found to be positive correlated. The DNA-synthesising part of the population shows characteristic differences to the mean value of volume and DNA-content of the remaining population. The factor circumference 2/area is suitable to characterize the differentiation degree of cells.
在幼年变态后的普通欧螈前肢截肢15天后，通过福尔根光度法和电子面积测定法，对厚度为2微米的纵切片中中胚层芽基细胞的细胞核DNA含量和体积进行了研究。在用氚标记的胸腺嘧啶核苷进行“体内”标记后，使用微放射自显影术对DNA合成期进行了鉴别。已发现核切片的体积与DNA含量呈正相关。群体中进行DNA合成的部分与其余群体的体积和DNA含量平均值表现出特征性差异。“周长2/面积”这一因子适用于表征细胞的分化程度。
