Anton H J, Elsen W
Microsc Acta Suppl. 1979(3):205-11.
Freezing dried semithin sections (1 micron) coloured with nuclear fast red are suited for determination of volume and activation state of cells and nuclei by means of electronic image analysis. Additional investigations of the proteinsynthesis with help of autoradiographs after incorporation of tritiated amino acids affirm the informations. Magnification of the nucleus together with a simultaneous increase of the proteinsynthesis per unit volume characterize the Stratum basale near the amputation level as the most active area of the regeneration epidermis. The cell volume is correlated with the alterations of the nuclear volume. Determination of the mean relative volume of nuclei with aid of measurements of nuclei sections suffices to get informations about cell activity.
用核固红染色的冻干半薄切片（1微米）适用于通过电子图像分析来测定细胞和细胞核的体积及活化状态。在掺入氚标记氨基酸后借助放射自显影片对蛋白质合成进行的进一步研究证实了这些信息。细胞核的增大以及单位体积内蛋白质合成的同时增加表明，截肢水平附近的基底层是再生表皮最活跃的区域。细胞体积与细胞核体积的变化相关。借助细胞核切片的测量来确定细胞核的平均相对体积，足以获取有关细胞活性的信息。
