马齿苋粉（Mesembryanthemum forsskalei Hochst. ex Boiss）中酚类和硫代葡萄糖苷化合物的液相色谱-电喷雾串联质谱分析及其对人血浆氧化应激的抑制作用

LC-ESI-MS/MS profile of phenolic and glucosinolate compounds in samh flour (Mesembryanthemum forsskalei Hochst. ex Boiss) and the inhibition of oxidative stress by these compounds in human plasma.

作者信息

Hamed Arafa I, Said Ridha Ben, Kontek Bogdan, Al-Ayed Abdullah S, Kowalczyk Mariusz, Moldoch Jaroslaw, Stochmal Anna, Olas Beata

机构信息

Phytochemistry Laboratory, Department of Botany, Faculty of Science, Aswan University, Aswan 81528, Egypt; Department of Chemistry, College of Science & Arts at Al-Rass, Qassim University, P.O. 53, Kingdom of Saudi Arabia; Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, ul. Czartoryskich 8, 24-100 Pulawy, Poland.

Unité Physico Chimie des Matériaux Condensés-UR11ES19, Département de Chimie, Faculté des Sciences de Tunis Université, Tunis El Manar Campus Universitaire, MANAR II, Tunis, Tunisia; Department of Chemistry, College of Science & Arts at Al-Rass, Qassim University, P.O. 53, Kingdom of Saudi Arabia.

出版信息

Food Res Int. 2016 Jul;85:282-290. doi: 10.1016/j.foodres.2016.04.009. Epub 2016 Apr 20.

DOI:10.1016/j.foodres.2016.04.009

PMID:29544846

Abstract

Samh flour (Mesembryanthemum forsskalei) is a foodstuff with high protein content, which can be used as a replacement for wheat flour. It is often consumed by Bedouin tribes of northern Saudi Arabia. Very little is known about bioactive molecules present in samh flour, therefore we analyzed its extracts to evaluate the contents of secondary metabolites. A total of 43 secondary metabolites present in 60% MeOH extract of samh flour were tentatively identified using LC-ESI-MS/MS. These compounds represented five major categories: glucosinolates, sinapic acid and sinapoylglycosides, acylated flavonoids, flavonoids, and amide derivatives. Their effect on oxidative damage of proteins and lipids was determined in vitro by assessing levels of protein thiol groups and concentrations of thiobarbituric acid reactive species (TBARS) in human plasma. Obtained results indicated that samh flour is a rich source of compounds with antioxidant activity.

摘要

萨姆面粉（Mesembryanthemum forsskalei）是一种蛋白质含量高的食品，可用作小麦粉的替代品。沙特阿拉伯北部的贝都因部落经常食用它。关于萨姆面粉中存在的生物活性分子知之甚少，因此我们分析了其提取物以评估次生代谢产物的含量。使用LC-ESI-MS/MS初步鉴定了萨姆面粉60%甲醇提取物中存在的43种次生代谢产物。这些化合物分为五大类：硫代葡萄糖苷、芥子酸和芥子酰糖苷、酰化黄酮类、黄酮类和酰胺衍生物。通过评估人血浆中蛋白质巯基水平和硫代巴比妥酸反应性物质（TBARS）浓度，在体外测定了它们对蛋白质和脂质氧化损伤的影响。所得结果表明，萨姆面粉是具有抗氧化活性化合物丰富的来源。