Grzegorczyk-Karolak Izabela, Krzemińska Marta, Kiss Anna K, Olszewska Monika A, Owczarek Aleksandra
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, 90-151 Lodz, Poland.
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 02-097 Warsaw, Poland.
Metabolites. 2020 Dec 3;10(12):497. doi: 10.3390/metabo10120497.
Plants have been used for medical purposes since ancient times. However, a detailed analysis of their biological properties and their associated active compounds is needed to justify their therapeutic use in modern medicine. The aim of the study was to identify and quantify the phenolics present in hydromethanolic extracts of the roots and shoots of the Chinese species, . The qualitative and quantitative analyses were carried out by ultrahigh-performance liquid chromatography with electrospray ionization mass spectrometry detection (UHPLC-PDA-ESI-MS), and high-performance liquid chromatography with photodiode array (HPLC-PDA) detection. The extracts of were also screened for their antioxidant activity using ferric ion (Fe) reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) cation (ABTS), superoxide radical anion (O), and inhibition of lipid peroxidation assays. The extracts were found to contain 38 substances, of which 36 were phenols, with a total level of 14.4 mg/g DW (dry weight) in shoots, and 23.1 mg/g DW in roots. Twenty-eight phenols were polyphenolic acids or their derivatives, the most abundant in shoots being rosmarinic acid, and in roots, salvianolic acid K followed by rosmarinic acid. The other major phenolic acids were caffeic acid, caffeoyl-threonic acids, isomers of lithospermic acid, salvianolic acid F, salvianolic acid B, and yunnaneic acid E. In addition to polyphenolic acids, nine flavonoids were detected in the shoot extract. While both extracts showed significant antioxidant activity, the shoot extract, containing both polyphenolic acids and flavonoids, demonstrated a slightly greater antioxidant potential in some of the anti-radical tests than the roots. However, the root extract proved to be slightly more effective in the lipid peroxidation inhibition test. Thus, was demonstrated as a promising source of antioxidants, and worthy of further more detailed studies.
自古以来，植物就被用于医疗目的。然而，需要对其生物学特性及其相关活性化合物进行详细分析，以证明它们在现代医学中的治疗用途。本研究的目的是鉴定和定量中国物种根和茎的氢甲醇提取物中存在的酚类物质。定性和定量分析通过超高效液相色谱-电喷雾电离质谱检测（UHPLC-PDA-ESI-MS）以及带有光电二极管阵列检测的高效液相色谱（HPLC-PDA）进行。还使用铁离子（Fe）还原抗氧化能力（FRAP）、1,1-二苯基-2-苦基肼（DPPH）、2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）二铵阳离子（ABTS）、超氧阴离子自由基（O）以及脂质过氧化抑制试验对提取物的抗氧化活性进行了筛选。发现提取物中含有38种物质，其中36种为酚类，茎中的总含量为14.4毫克/克干重（DW），根中的含量为23.1毫克/克DW。28种酚为多酚酸或其衍生物，茎中含量最丰富的是迷迭香酸，根中是丹酚酸K，其次是迷迭香酸。其他主要酚酸为咖啡酸、咖啡酰苏糖酸、紫草酸异构体、丹酚酸F、丹酚酸B和云南酸E。除了多酚酸外，在茎提取物中还检测到9种黄酮类化合物。虽然两种提取物都显示出显著的抗氧化活性，但含有多酚酸和黄酮类化合物的茎提取物在一些抗自由基试验中表现出比根提取物略高的抗氧化潜力。然而，根提取物在脂质过氧化抑制试验中被证明略为有效。因此，该物种被证明是一种有前景的抗氧化剂来源，值得进一步进行更详细的研究。
