Morii N, Nakao K, Itoh H, Shiono S, Yamada T, Sugawara A, Saito Y, Mukoyama M, Arai H, Sakamoto M
Biochem Biophys Res Commun. 1987 May 29;145(1):196-203. doi: 10.1016/0006-291x(87)91306-4.
Using a radioimmunoassay for alpha-rat atrial natriuretic polypeptide (alpha-rANP), tissue levels of alpha-rANP-like immunoreactivity (-LI) in the rat spinal cord and autonomic ganglia were investigated. The alpha-rANP-LI level was higher in the more caudal parts of the spinal cord and the highest in the sacral spinal cord. alpha-rANP-LI was also detected in the superior cervical and coeliac ganglia. Gel permeation chromatographic analysis showed that the major peak of alpha-rANP-LI in the spinal cord was a low molecular weight form co-eluted with synthetic alpha-rANP. Reverse-phase high performance liquid chromatographic analysis revealed that alpha-rANP-LI with a low molecular weight in the spinal cord consisted of several components, two major components of which co-migrated with synthetic alpha-rANP (4-28) and alpha-rANP (5-28), whereas little immunoreactivity was eluted at the position of alpha-rANP. These findings suggest the involvement of ANP in the function of the spinal cord and autonomic nervous system.
利用放射免疫分析法检测α-大鼠心房利钠多肽（α-rANP），对大鼠脊髓和自主神经节中α-rANP样免疫反应性（-LI）的组织水平进行了研究。α-rANP-LI水平在脊髓较尾端部分较高，在骶脊髓中最高。在颈上神经节和腹腔神经节中也检测到了α-rANP-LI。凝胶渗透色谱分析表明，脊髓中α-rANP-LI的主要峰是与合成α-rANP共洗脱的低分子量形式。反相高效液相色谱分析显示，脊髓中低分子量的α-rANP-LI由几个组分组成，其中两个主要组分与合成α-rANP（4-28）和α-rANP（5-28）共迁移，而在α-rANP位置洗脱的免疫反应性很少。这些发现提示心房利钠肽参与脊髓和自主神经系统的功能。
