Sakamoto M, Nakao K, Kihara M, Morii N, Sugawara A, Suda M, Shimokura M, Kiso Y, Yamori Y, Imura H
Biochem Biophys Res Commun. 1985 May 16;128(3):1281-7. doi: 10.1016/0006-291x(85)91079-4.
Using a specific radioimmunoassay (RIA) for alpha-atrial natriuretic polypeptide (alpha-ANP), we have demonstrated the presence of alpha-rat ANP-like immunoreactivity (alpha-rANP-LI) in the rat kidney which is considered to be a target organ for atrial natriuretic polypeptides released from the heart. Most of alpha-rANP-LI was localized in the cortex. High performance gel permeation chromatography coupled with the RIA revealed that renal alpha-rANP-LI was eluted at the position of a low molecular weight form corresponding to alpha-rANP without detectable amounts of high molecular weight forms. This is in contrast to the observation that gamma-rANP, a high molecular weight form of 13k daltons, is the dominant form of alpha-rANP-LI in the rat atrium. In water-deprived rats, the concentration and content of alpha-rANP-LI in the kidney showed a significant decrease compared with control rats. In addition, the alpha-rANP-LI concentration and content in this organ revealed a substantial decrease after perfusion with physiological saline. These results indicate the existence of atrial natriuretic polypeptide (ANP) in the kidney and suggest that part of renal ANP may originate from the heart.
使用一种针对α-心房利钠多肽（α-ANP）的特异性放射免疫测定法（RIA），我们已证实在大鼠肾脏中存在α-大鼠ANP样免疫反应性（α-rANP-LI），而肾脏被认为是从心脏释放的心房利钠多肽的靶器官。大部分α-rANP-LI定位于皮质。高效凝胶渗透色谱结合RIA显示，肾脏中的α-rANP-LI在对应于α-rANP的低分子量形式的位置被洗脱，未检测到高分子量形式。这与观察结果相反，即13千道尔顿的高分子量形式γ-rANP是大鼠心房中α-rANP-LI的主要形式。在缺水大鼠中，与对照大鼠相比，肾脏中α-rANP-LI的浓度和含量显著降低。此外，用生理盐水灌注后，该器官中的α-rANP-LI浓度和含量大幅下降。这些结果表明肾脏中存在心房利钠多肽（ANP），并提示肾脏中的部分ANP可能源自心脏。
