Shiono S, Nakao K, Morii N, Yamada T, Itoh H, Sakamoto M, Sugawara A, Saito Y, Katsuura G, Imura H
Biochem Biophys Res Commun. 1986 Mar 28;135(3):728-34. doi: 10.1016/0006-291x(86)90989-7.
Using reverse phase high performance liquid chromatography (RP-HPLC) coupled with two radioimmunoassays for atrial natriuretic polypeptide (ANP) with different specificities, we investigated the nature of alpha-rat ANP-like immunoreactivity (alpha-rANP-LI) with a low molecular weight in the rat brain. Two major peaks with alpha-rANP-LI in the extract from rat whole brains were eluted in the vicinity of the elution position of synthetic alpha-rANP, a 28-amino acid polypeptide. These two components co-migrated with synthetic alpha-rANP (4-28) and alpha-rANP (5-28), respectively. The peak corresponding to alpha-rANP (4-28) was the highest, and only a little alpha-rANP-LI was detected at the elution position of alpha-rANP. An identical profile in RP-HPLC was also observed in the extract from the rat hypothalamus. These results indicate that the major components of alpha-rANP-LI with a low molecular weight in the rat brain are alpha-rANP (4-28) and alpha-rANP (5-28).
利用反相高效液相色谱法（RP-HPLC）结合两种针对心房利钠多肽（ANP）的具有不同特异性的放射免疫分析法，我们研究了大鼠脑中低分子量的α-大鼠ANP样免疫反应性（α-rANP-LI）的性质。在大鼠全脑提取物中，具有α-rANP-LI的两个主要峰在合成α-rANP（一种28个氨基酸的多肽）的洗脱位置附近被洗脱出来。这两个成分分别与合成α-rANP（4-28）和α-rANP（5-28）共同迁移。对应于α-rANP（4-28）的峰最高，并且在α-rANP的洗脱位置仅检测到少量的α-rANP-LI。在大鼠下丘脑提取物中也观察到了RP-HPLC中的相同图谱。这些结果表明，大鼠脑中低分子量的α-rANP-LI的主要成分是α-rANP（4-28）和α-rANP（5-28）。
