Tang Luyan, Wu Wenyu, Fu Wenwen, Hu Yao
Department of Dermatology, Huashan Hospital of Fudan University, Shanghai 200040, P.R. China.
Exp Ther Med. 2018 Apr;15(4):3459-3466. doi: 10.3892/etm.2018.5807. Epub 2018 Jan 30.
Phototherapy is widely used in the treatment of vitiligo. Previous studies have focused on the effects of ultraviolet (UV) radiation on melanocytes; however, the biological effects of phototherapy and melanocytes on keratinocytes remain to be elucidated. To investigate and assess the effects of clinically doses of broad band (BB)-UVA, narrow band (NB)-UVB and melanocytes on human keratinocytes , clinical doses of BB-UVA or NB-UVB radiation and human melanoma cell A375 co-culture were performed as stress divisors to HaCaT cells. Cell proliferation, expression of protease-activated receptor-2 (PAR-2) and nuclear factor E2-related factor 2 mRNA, lipid peroxidation and intracellular antioxidant level of keratinocytes were analyzed. It was demonstrated that UV radiation inhibited the proliferation of cells apart from following exposure to low dose (1 J/cm) UVA. Medium dose (5 J/cm) UVA radiation had no adverse effects on lipid peroxidation and increased antioxidant levels in HaCaT cells. Medium (200 mJ/cm) and high (400 mJ/cm) doses of UVB radiation induced cellular damage due to increased lipid peroxidation as indicated by levels of malondialdehyde. Furthermore, A375 co-culture treatment induced a similar effect on the lipid peroxidation of HaCaT as with low dose UVB radiation. Therefore, the results of the present study determined that clinical doses of BB-UVA and NB-UVB radiation had varying effects on proliferation and related protein levels in HaCaT cells. Co-culture with A375 had similar effects as those of low dose UVA and UVB radiation, in which the PAR-2 expression was significantly upregulated.
光疗广泛应用于白癜风的治疗。以往的研究主要集中在紫外线（UV）辐射对黑素细胞的影响；然而，光疗和黑素细胞对角质形成细胞的生物学作用仍有待阐明。为了研究和评估临床剂量的宽带（BB）-UVA、窄带（NB）-UVB和黑素细胞对人角质形成细胞的影响，将临床剂量的BB-UVA或NB-UVB辐射与人黑色素瘤细胞A375共培养作为对HaCaT细胞的应激因素。分析了角质形成细胞的细胞增殖、蛋白酶激活受体-2（PAR-2）和核因子E2相关因子2 mRNA的表达、脂质过氧化和细胞内抗氧化水平。结果表明，除了低剂量（1 J/cm）UVA照射外，UV辐射抑制细胞增殖。中等剂量（5 J/cm）UVA辐射对HaCaT细胞的脂质过氧化没有不良影响，并提高了抗氧化水平。中等剂量（200 mJ/cm）和高剂量（400 mJ/cm）的UVB辐射由于丙二醛水平升高导致脂质过氧化增加，从而诱导细胞损伤。此外，A375共培养处理对HaCaT细胞的脂质过氧化产生了与低剂量UVB辐射类似的影响。因此，本研究结果确定临床剂量的BB-UVA和NB-UVB辐射对HaCaT细胞的增殖和相关蛋白水平有不同影响。与A375共培养产生的影响与低剂量UVA和UVB辐射类似，其中PAR-2表达显著上调。
