Sheibani K, Nathwani B N, Winberg C D, Burke J S, Swartz W G, Blayney D, van de Velde S, Hill L R, Rappaport H
Cancer. 1987 Jul 15;60(2):183-90. doi: 10.1002/1097-0142(19870715)60:2<183::aid-cncr2820600211>3.0.co;2-0.
A large panel of monoclonal antibodies and polyclonal antisera were used to ascertain the immunophenotypic characteristics of 36 lymphoblastic lymphomas (LBL). Results showed that this group of lymphomas have significant immunologic heterogeneity. Of the 36 cases, 33 were positive for T-cell antigens; among these, 22 cases were classified as T-cell LBL (TLBL, Group 1) based on their expression of T-cell-restricted and T-cell-associated antigens, and five expressed the common acute lymphoblastic leukemia antigen in addition to T-cell-associated antigens (Group 2). Six cases showed strong reactivity with anti-Leu-11 antibody, which defines a specific subtype of lymphocytes considered to have a natural killer (NK) function (Group 3). Two additional cases had a "pre-B" cell phenotype (Group 4), as determined by reactivity with BA-1 and BA-2 monoclonal antibodies, which react with immature and pre-B-lymphocytes. The neoplastic cells in the remaining case showed monoclonal surface membrane immunoglobulin of the IgMD heavy chain and kappa light chain type (Group 5). Despite immunophenotypic heterogeneity, the morphologic features were essentially similar in all cases. When the clinical features for each immunologic group were compared, however, two statistically significant findings resulted: (1) the frequency of mediastinal masses was highest in TLBL (Group 1, P less than 0.01), and (2) the male-female ratio was significantly lower in patients with LBL expressing NK-associated antigens (Group 3) than in the other groups of patients (P less than 0.01). Our data indicate that LBL can be divided into several immunologic subtypes; larger, prospective clinicopathologic studies are required to determine the clinical significance of the immunophenotypic classifications of LBL.
使用一大组单克隆抗体和多克隆抗血清来确定36例淋巴母细胞淋巴瘤（LBL）的免疫表型特征。结果显示，该组淋巴瘤具有显著的免疫异质性。在这36例病例中，33例T细胞抗原呈阳性；其中，22例基于其T细胞限制性和T细胞相关抗原的表达被分类为T细胞LBL（TLBL，第1组），5例除T细胞相关抗原外还表达常见急性淋巴细胞白血病抗原（第2组）。6例与抗Leu-11抗体表现出强反应性，抗Leu-11抗体可定义一种被认为具有自然杀伤（NK）功能的淋巴细胞特定亚型（第3组）。另外2例具有“前B”细胞表型（第4组），这是通过与BA-1和BA-2单克隆抗体的反应性确定的，BA-1和BA-2单克隆抗体可与未成熟和前B淋巴细胞发生反应。其余病例中的肿瘤细胞显示为IgM重链和κ轻链型的单克隆表面膜免疫球蛋白（第5组）。尽管存在免疫表型异质性，但所有病例的形态学特征基本相似。然而，当比较每个免疫组的临床特征时，得出了两个具有统计学意义的结果：（1）纵隔肿块的发生率在TLBL中最高（第1组，P<0.01），（2）表达NK相关抗原的LBL患者（第3组）的男女比例显著低于其他组患者（P<0.01）。我们的数据表明，LBL可分为几种免疫亚型；需要进行更大规模的前瞻性临床病理研究来确定LBL免疫表型分类的临床意义。
