Noulsri Egarit, Abudaya Dinar, Lerdwana Surada, Pattanapanyasat Kovit
Research Division, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Immunology, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Lab Med. 2018 Jul 5;49(3):246-253. doi: 10.1093/labmed/lmy002.
To determine whether a corrected lymphocyte percentage could reduce bias in the absolute cluster of differentiation (CD)4+ T lymphocyte counts obtained via dual-platform (DP) vs standard single-platform (SP) flow cytometry.
The correction factor (CF) for the lymphocyte percentages was calculated at 6 laboratories. The absolute CD4+ T lymphocyte counts in 300 blood specimens infected with human immunodeficiency virus (HIV) were determined using the DP and SP methods.
Applying the CFs revealed that 4 sites showed a decrease in the mean bias of absolute CD4+ T lymphocyte counts determined via DP vs standard SP (-109 vs -84 cells/μL, -80 vs -58 cells/μL, -52 vs -45 cells/μL, and -32 vs 1 cells/μL). However, 2 participating laboratories revealed an increase in the difference of the mean bias (-42 vs -49 cells/μL and -20 vs -69 cells/μL).
Use of the corrected lymphocyte percentage shows potential for decreasing the difference in CD4 counts between DP and the standard SP method.
确定校正后的淋巴细胞百分比是否能减少通过双平台（DP）与标准单平台（SP）流式细胞术获得的绝对分化簇（CD）4+T淋巴细胞计数中的偏差。
在6个实验室计算淋巴细胞百分比的校正因子（CF）。使用DP和SP方法测定300份感染人类免疫缺陷病毒（HIV）的血液标本中的绝对CD4+T淋巴细胞计数。
应用CFs显示，4个位点显示通过DP与标准SP测定的绝对CD4+T淋巴细胞计数的平均偏差有所降低（-109对-84细胞/μL、-80对-58细胞/μL、-52对-45细胞/μL、-32对1细胞/μL）。然而，2个参与实验室显示平均偏差差异增加（-42对-49细胞/μL和-20对-69细胞/μL）。
使用校正后的淋巴细胞百分比显示出减少DP与标准SP方法之间CD4计数差异的潜力。
