Liu Jie, Song Yi, Lu Xiaoguang, Chen Tuo, Guo Wenxiu, Fan Zhiwei, Kang Xin, Wang Yu, Wang Yi
Emergency Department, Affiliated Zhongshan Hospital of Dalian University, Dalian No. 6, Jiefang Street, Zhongshan District, Dalian, 116001, Liaoning, China.
Graduate School, Zunyi Medical College, Zunyi, 563003, China.
Curr Microbiol. 2018 Aug;75(8):1006-1010. doi: 10.1007/s00284-018-1480-6. Epub 2018 Mar 15.
Recently, several studies have indicated that the intestinal microbiota can be regulated by the individual attributes, and even the alternation of circadian rhythm. Inspired by this, we speculated that seasonal variation might also have some effect on the intestinal microbiota. A total of 11 Sprague-Dawley male rats, weighing 250-280 g, were divided into summer group (n = 5) and winter group (n = 6). Cages were individually ventilated at 20 ± 2 °C and 45-65% relative humidity with a circadian rhythm of 12/12 h. After 1 week of adaptively feeding, mucosal contents of jejunum, terminal ileum, and ascending colon were collected and analyzed by 16S rRNA Gene Amplicon Pyrosequencing. The results showed that intestinal microbiota of rats for the same strain were affected by season change under the same feeding condition and living environment. We should take seasonal factor into account in the future experimental design based on intestinal microbiome.
最近，多项研究表明，肠道微生物群可受个体属性甚至昼夜节律变化的调节。受此启发，我们推测季节变化可能也会对肠道微生物群产生一定影响。将11只体重250 - 280克的雄性Sprague-Dawley大鼠分为夏季组（n = 5）和冬季组（n = 6）。将笼子单独通风，温度保持在20±2°C，相对湿度为45 - 65%，昼夜节律为12/12小时。适应性喂养1周后，收集空肠、回肠末端和升结肠的黏膜内容物，并通过16S rRNA基因扩增焦磷酸测序进行分析。结果表明，在相同的喂养条件和生活环境下，同一品系大鼠的肠道微生物群会受到季节变化的影响。在未来基于肠道微生物组的实验设计中，我们应考虑季节因素。
