Wise Laboratory of Environmental and Genetic Toxicology, Department of Pharmacology and Toxicology, University of Louisville, CTRB rm 522, 505 S. Hancock Street, Louisville, Kentucky, 40292, USA.
Wise Laboratory of Environmental and Genetic Toxicology, Department of Pharmacology and Toxicology, University of Louisville, CTRB rm 522, 505 S. Hancock Street, Louisville, Kentucky, 40292, USA; Toxicology Program, Department of Biological Sciences, North Carolina State University, Box 7633, Raleigh NC 27695-7633, 850 Main Campus Drive, Raleigh, NC 27606, USA.
Aquat Toxicol. 2018 May;198:149-157. doi: 10.1016/j.aquatox.2018.03.003. Epub 2018 Mar 4.
Hexavalent chromium [Cr(VI)] is a marine pollution of concern as recent studies show it has a global distribution, with some regions showing high Cr concentrations in marine animal tissue, and it is extensively used. Leatherback sea turtles (Dermochelys coriacea) are an endangered marine species that may experience prolonged exposures to environmental contaminants including Cr(VI). Human activities have led to global Cr(VI) contamination of the marine environment. While Cr(VI) has been identified as a known human carcinogen, the health effects in marine species are poorly understood. In this study, we assessed the cytotoxic and genotoxic effects of particulate and soluble Cr(VI) in leatherback sea turtle lung cells. Both particulate and soluble Cr(VI) induced a concentration-dependent increase in cytotoxicity. Next, using a chromosome aberration assay, we assessed the genotoxic effects of Cr(VI) in leatherback sea turtle lung cells. Particulate and soluble Cr(VI) induced a concentration-dependent increase in clastogenicity in leatherback sea turtle lung cells. These data indicate that Cr(VI) may be a health concern for leatherback sea turtles and other long-lived marine species. Additionally, these data provide foundational support to use leatherback sea turtles as a valuable model species for monitoring the health effects of Cr(VI) in the environment and possibly as an indicator species to assess environmental human exposures and effects.
六价铬[Cr(VI)]是一种海洋污染物，因为最近的研究表明它在全球范围内分布，一些地区的海洋动物组织中显示出高浓度的 Cr，并且它被广泛使用。棱皮龟（Dermochelys coriacea）是一种濒危的海洋物种，可能会长期暴露于包括 Cr(VI)在内的环境污染物中。人类活动导致了全球海洋环境中 Cr(VI)的污染。虽然 Cr(VI)已被确定为已知的人类致癌物，但海洋物种的健康影响仍知之甚少。在这项研究中，我们评估了粒状和可溶性 Cr(VI)对棱皮龟肺细胞的细胞毒性和遗传毒性作用。粒状和可溶性 Cr(VI)均诱导细胞毒性呈浓度依赖性增加。接下来，我们使用染色体畸变试验评估了 Cr(VI)对棱皮龟肺细胞的遗传毒性作用。粒状和可溶性 Cr(VI)诱导棱皮龟肺细胞的断裂诱导作用呈浓度依赖性增加。这些数据表明，Cr(VI)可能是棱皮龟和其他长寿海洋物种的健康隐患。此外，这些数据为使用棱皮龟作为监测环境中 Cr(VI)对健康影响的有价值的模型物种提供了基础支持，并可能作为评估环境中人类暴露和影响的指示物种。
