Theiss G, John A, Morich F, Neuser D, Schröder W, Stasch J P, Wohlfeil S
FEBS Lett. 1987 Jun 22;218(1):159-62. doi: 10.1016/0014-5793(87)81038-4.
Alpha-human atrial natriuretic peptide (alpha-h-ANP) was purified to homogeneity from human plasma of healthy adults in a three-step procedure including immunoaffinity chromatography on immobilized monoclonal anti-alpha-h-ANP antibody (moab). A single peak of immunoreactivity was obtained after final reversed-phase HPLC and the amino acid sequence of the isolated material was identical to that of synthetic alpha-h-ANP. No further atrial peptides could be detected although the moab reacts with all biologically active peptides. It is therefore concluded that alpha-h-ANP is the only form of ANP circulating in human plasma.
通过三步法从健康成年人的人血浆中纯化出α-人心房利钠肽（α-h-ANP）至同质，这三步包括在固定化单克隆抗α-h-ANP抗体（moab）上进行免疫亲和色谱。最终的反相高效液相色谱后获得了一个单一的免疫反应峰，且分离出的物质的氨基酸序列与合成的α-h-ANP相同。尽管该单克隆抗体与所有生物活性肽都有反应，但未检测到其他心房肽。因此得出结论，α-h-ANP是人类血浆中循环的ANP的唯一形式。
