Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan; Division of infectious Diseases Epidemiology and Control, National Defense Medical Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Virus Res. 2018 Apr 2;249:57-65. doi: 10.1016/j.virusres.2018.03.004. Epub 2018 Mar 13.
The genus Thogotovirus, as represented by Thogoto virus and Dhori virus, comprises a group of arthropod-borne viruses, most members of which are transmitted by ticks. Here we report the genetic and biological characterization of a new thogotovirus, designated Oz virus (OZV), isolated from the hard tick Amblyomma testudinarium in Ehime, Japan. OZV efficiently replicated and induced a cytopathic effect in Vero cells, from which enveloped pleomorphic virus particles were formed by budding. OZV could also replicate in BHK-21 and DH82 cells and caused high mortality in suckling mice after intracerebral inoculation. Phylogenetic analyses of six viral proteins indicated that OZV is clustered with Dhori and related viruses, and is most closely related in glycoprotein (GP) and matrix protein (M) sequences to Bourbon virus, a human-pathogenic thogotovirus discovered recently in the United States. Our findings emphasize the need for understanding the geographic distribution and ecology of OZV and related viruses and for reevaluation of the medical and public health importance of thogotoviruses.
该 Thogotovirus 属，由 Thogoto 病毒和 Dhori 病毒代表，包括一组节肢动物传播的病毒，其中大多数成员通过蜱传播。在这里，我们报告了一种新的 Thogotovirus，命名为 Oz 病毒（OZV），它是从日本爱媛县的硬蜱 Amblyomma testudinarium 中分离出来的。OZV 在 Vero 细胞中高效复制并诱导细胞病变效应，通过出芽形成包膜的多形病毒颗粒。OZV 还可以在 BHK-21 和 DH82 细胞中复制，并在脑内接种后导致幼鼠高死亡率。六种病毒蛋白的系统发育分析表明，OZV 与 Dhori 和相关病毒聚类，在糖蛋白（GP）和基质蛋白（M）序列上与 Bourbon 病毒最密切相关，Bourbon 病毒是最近在美国发现的一种人类致病性 Thogotovirus。我们的研究结果强调了需要了解 OZV 和相关病毒的地理分布和生态学，并重新评估 Thogotoviruses 的医学和公共卫生重要性。
