Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.
Institute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.
J Nat Med. 2022 Jan;76(1):39-48. doi: 10.1007/s11418-021-01546-6. Epub 2021 Aug 3.
25 phenolic acids, including four new isolates, eurylophenosides A-D (1-4) and 21 known ones (5-25) were isolated and identified from the stems of Oplopanax elatus Nakai. Among the known compounds 5-9, 11-13, 16, 18-25 were isolated from the genus for the first time; 17 was first obtained from the plant; and the NMR data of 22 was reported here first. Meanwhile, the UVB-induced photodamage model of HaCaT cells was used to study the prevent-photodamage abilities of compounds 1-2, 4-8, 11-13 and 15-25 with a nontoxic concentration at 50 μM. Moreover, a dose-dependent experiment was conducted for active compounds at the concentration of 10, 25, and 50 µM, respectively. Consequently, pretreatment with compounds 1, 16, 17, 19, 20, 22, 24 and 25 could suppress the cell viability decreasing induced by UVB irradiation in a concentration-dependent manner. These results indicated that phenolic acids were one kind of material basis with prevent-photodamage activity of O. elatus.
从杠柳的茎中分离并鉴定了 25 种酚酸，包括 4 种新的分离物 eurylophenosides A-D（1-4）和 21 种已知的酚酸（5-25）。在已知的化合物 5-9、11-13、16、18-25 中，有 5-9、11-13、16 是首次从该属中分离得到的；17 是首次从该植物中分离得到的；22 的 NMR 数据首次在此报道。同时，采用 HaCaT 细胞 UVB 诱导的光损伤模型，以 50 μM 的无毒浓度研究了化合物 1-2、4-8、11-13 和 15-25 对化合物的预防光损伤能力。此外，在浓度为 10、25 和 50 μM 时，对活性化合物进行了剂量依赖性实验。结果表明，化合物 1、16、17、19、20、22、24 和 25 预处理能够浓度依赖性地抑制 UVB 照射诱导的细胞活力降低。这些结果表明，酚酸是杠柳具有预防光损伤活性的一种物质基础。
