Nguyen Van Phuc, Zhu Tianye, Henry Jessica, Zhang Wei, Wang Xueding, Paulus Yannis M
Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48105, USA.
Photonics. 2022 Mar;9(3). doi: 10.3390/photonics9030201. Epub 2022 Mar 21.
Photoacoustic microscopy (PAM) is an emerging retinal imaging technique that can provide high spatial resolution and high contrast of chorioretinal vessels. PAM is compatible with optical coherence tomography (OCT) and fluorescence imaging, allowing for development of a multimodal imaging system that combines these imaging modalities into one. This study presents a non-invasive, label-free in vivo imaging of retinal and choroidal vascular occlusion using multimodal imaging system, including PAM and OCT. Both retinal vein occlusion (RVO) and choroidal vascular occlusion (CVO) were clearly identified selectively using a spectroscopic PAM imaging. RVO and CVO were created in six rabbits using laser photocoagulation. The dynamic changes of retinal vasculature were observed and evaluated using color fundus photography, fluorescein angiography, OCT, and PAM. The position of RVO and CVO were imaged with different wavelengths ranging from 532 to 600 nm. The data shows that occluded vessels were clearly distinguished from the surrounding retinal vessels on the PAM images. This advanced imaging system is a promising technique for imaging retinal ischemia in preclinical disease models.
光声显微镜（PAM）是一种新兴的视网膜成像技术，能够提供脉络膜视网膜血管的高空间分辨率和高对比度成像。PAM与光学相干断层扫描（OCT）和荧光成像兼容，有助于开发一种将这些成像方式整合为一体的多模态成像系统。本研究使用包括PAM和OCT在内的多模态成像系统，展示了对视网膜和脉络膜血管阻塞进行非侵入性、无标记的体内成像。通过光谱PAM成像能够选择性地清晰识别视网膜静脉阻塞（RVO）和脉络膜血管阻塞（CVO）。使用激光光凝在六只兔子身上制造了RVO和CVO。利用彩色眼底照相、荧光素血管造影、OCT和PAM观察并评估视网膜血管的动态变化。在532至600nm的不同波长下对RVO和CVO的位置进行成像。数据表明，在PAM图像上，阻塞血管与周围视网膜血管能够清晰区分。这种先进的成像系统是在临床前疾病模型中对视网膜缺血进行成像的一种有前景的技术。
