Kirkland David, Fowler Paul
Kirkland Consulting, Tadcaster, UK.
FSTox Consulting Ltd., Raunds, UK.
Mutat Res Genet Toxicol Environ Mutagen. 2018 Apr;828:36-45. doi: 10.1016/j.mrgentox.2018.02.006. Epub 2018 Feb 26.
Trimethylolpropane triacrylate (TMPTA) is a trifunctional acrylate monomer which polymerizes rapidly when exposed to sources of free radicals. It is widely used as a reactive diluent and polymer building block in the formulation of overprint varnishes, inks and a variety of wood, plastic and metal coatings. TMPTA has been tested in a range of in vitro and in vivo genotoxicity tests. There is no clear evidence of induction of gene mutations by TMPTA in bacteria or mammalian cells in vitro, but there is evidence of clastogenicity from induction of small colony tk mutants in the mouse lymphoma assay, and also induction of micronuclei and chromosomal aberrations. However, TMPTA was negative in bone marrow or blood micronucleus tests in vivo following oral or repeated dermal application, and did not induce comets in bone marrow or liver of mice following intravenous administration, which would have achieved plasma (and therefore tissue) concentrations estimated to exceed those inducing clastogenic effects in vitro. It is concluded that TMPTA is not genotoxic in vivo.
三羟甲基丙烷三丙烯酸酯（TMPTA）是一种三官能丙烯酸酯单体，当暴露于自由基源时会迅速聚合。它被广泛用作罩光清漆、油墨以及各种木材、塑料和金属涂料配方中的反应性稀释剂和聚合物结构单元。TMPTA已在一系列体外和体内遗传毒性试验中进行了测试。没有明确证据表明TMPTA在体外细菌或哺乳动物细胞中诱导基因突变，但在小鼠淋巴瘤试验中有证据表明诱导小集落tk突变体具有致断裂性，并且还诱导微核和染色体畸变。然而，口服或重复经皮给药后，TMPTA在体内骨髓或血液微核试验中呈阴性，静脉给药后在小鼠骨髓或肝脏中未诱导彗星形成，而静脉给药可使血浆（进而组织）浓度达到估计超过体外诱导致断裂效应的浓度。结论是TMPTA在体内无遗传毒性。
