Luciani Mirella, Di Febo Tiziana, Orsini Massimiliano, Krasteva Ivanka, Cattaneo Angela, Podaliri Vulpiani Michele, Di Pancrazio Chiara, Bachi Angela, Tittarelli Manuela
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.
Istituto FIRC di Oncologia Molecolare (IFOM), Milan, Italy.
Front Vet Sci. 2018 Mar 5;5:40. doi: 10.3389/fvets.2018.00040. eCollection 2018.
The diagnosis of dourine can be difficult because the clinical signs of this disease in horses are similar to those of surra, caused by . Moreover, and are closely related and, so far, they cannot be distinguished using serological tests. In a previous work, the protein pattern recognized by antibodies from dourine-infected horses and the humoral immune response kinetics were investigated by immunoblotting assay; a total of 20 sera from naturally and experimentally infected horses and from healthy animals were tested. Immunoblotting analysis showed that antibodies from infected horses specifically bind low molecular weight proteins (from 16 to 35 kDa), which are not recognized by antibodies from uninfected horses. In this work, we tested other 615 sera (7 from naturally infected horses and 608 sera from healthy horses and donkeys): results confirmed the data obtained previously. In addition, six SDS-PAGE bands with molecular weight ranging from 10 to 37 kDa were analyzed by mass spectrometry, in order to identify immunogenic proteins that could be used as biomarkers for the diagnosis of dourine. A total of 167 proteins were identified. Among them, 37 were found unique for . Twenty-four of them could represent possible candidate diagnostic antigens for the development of serological tests specific for .
马媾疫的诊断可能具有挑战性，因为马匹中这种疾病的临床症状与锥虫病（由……引起）的症状相似。此外，（此处原文缺失相关内容）与（此处原文缺失相关内容）密切相关，并且到目前为止，无法通过血清学检测将它们区分开来。在先前的一项工作中，通过免疫印迹分析研究了马媾疫感染马匹的抗体所识别的蛋白质模式以及体液免疫反应动力学；总共检测了来自自然感染和实验感染马匹以及健康动物的20份血清。免疫印迹分析表明，感染马匹的抗体特异性结合低分子量蛋白质（16至35 kDa），未感染马匹的抗体无法识别这些蛋白质。在这项工作中，我们检测了其他615份血清（7份来自自然感染马匹，608份来自健康马匹和驴）：结果证实了先前获得的数据。此外，通过质谱分析了6条分子量范围为10至37 kDa的SDS-PAGE条带，以鉴定可作为马媾疫诊断生物标志物的免疫原性蛋白质。总共鉴定出167种蛋白质。其中，37种被发现是马媾疫特有的。其中24种可能代表用于开发马媾疫特异性血清学检测的潜在候选诊断抗原。
