UMR Santé et Agroécologie du Vignoble (1065), ISVV, Labex Cote, CS 20032 , INRA , 33882 Villenave d'Ornon , France.
Faculté des Sciences Pharmaceutiques, Unité de Recherche Œnologie EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (GESVAB), Institut des Sciences de la Vigne et du Vin, CS 50008 , Université de Bordeaux , 33882 Villenave d'Ornon , France.
J Agric Food Chem. 2018 Apr 4;66(13):3338-3350. doi: 10.1021/acs.jafc.7b05725. Epub 2018 Mar 26.
Grapevine is subject to diseases that affect yield and wine quality caused by various pathogens including Botrytis cinerea. To limit the use of fungicides, an alternative is to use plant elicitors such as benzothiadiazole (BTH). We investigated the effect of a fungicide (Pyrimethanil) and an elicitor (benzothiadiazole) on plant defenses. Applications for two consecutive years in the vineyard significantly reduced gray mold. Two and seven days after treatments, the expressions of 48 genes involved in defenses showed differential modulation (up- or down-regulation) depending on treatment. Some genes were identified as potential markers of protection and were linked to an increase in total polyphenols (TP) in leaves. Surprisingly, the fungicide also induced the expression of defense genes and increased the polyphenol content. This suggests that BTH acts as an efficient elicitor in the vineyard and that Pyrimethanil may act, in part, as a defense-inducing agent on the vine.
葡萄藤易患多种病原菌（包括灰葡萄孢菌）引起的病害，从而影响产量和葡萄酒的质量。为了减少杀菌剂的使用，可以选择使用植物激发子（如苯并噻二唑，BTH）。本研究调查了杀菌剂（咯菌腈）和激发子（苯并噻二唑）对植物防御的影响。在葡萄园连续两年使用后，灰霉病显著减少。处理后 2 天和 7 天，与处理相关的 48 种防御相关基因的表达出现差异调节（上调或下调）。一些基因被鉴定为潜在的保护标记物，并与叶片中总多酚（TP）的增加有关。令人惊讶的是，杀菌剂也诱导了防御基因的表达，并增加了多酚的含量。这表明 BTH 在葡萄园作为一种有效的激发子，咯菌腈可能部分作为一种诱导剂在葡萄藤上起作用。
