Univ. Bordeaux, Faculté des Sciences Pharmaceutiques, Unité de recherche Œnologie, EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique (GESVAB), ISVV, 33882, Villenave d'Ornon cedex, France.
Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, ul. Miecznikowa 1, 02-096, Warsaw, Poland.
Metabolomics. 2019 Apr 27;15(5):67. doi: 10.1007/s11306-019-1530-5.
Grapevine protection is an important issue in viticulture. To reduce pesticide use, sustainable disease control strategies are proposed, including a promising alternative method based on the elicitor-triggered stimulation of the grapevine natural defense responses. However, detailed investigations are necessary to characterize the impact of such defense induction on the primary metabolism.
Our aim was to use a metabolomics approach to assess the impact on grapevine of different elicitors dependent on the salicylic acid (SA) and/or jasmonic acid (JA) pathway. For this purpose, leaves of grapevine foliar cuttings were treated with methyl jasmonate, acibenzolar-S-methyl or phosphonates.
According to the elicitor, common and discriminating metabolites were elucidated using H NMR measurements and principal component analysis.
A wide range of compounds including carbohydrates, amino acids, organic acids, phenolics and amines were identified. The score plots obtained by combining PC1 versus PC2 and PC1 versus PC3 allowed a clear separation of samples, so metabolite fingerprinting showed an extensive reprogramming of primary metabolic pathways after elicitation.
The methods applied were found to be accurate for the rapid determination and differential characterization of plant samples based on their metabolic composition. These investigations can be very useful because the application of plant defense stimulators is gaining greater importance as an alternative strategy to pesticides in the vineyard.
葡萄藤保护是葡萄栽培中的一个重要问题。为了减少农药的使用，提出了可持续的疾病控制策略，包括一种有前途的替代方法，即基于激发葡萄藤天然防御反应的诱导剂。然而，有必要进行详细的调查，以描述这种防御诱导对初级代谢的影响。
我们的目的是使用代谢组学方法来评估不同诱导剂对葡萄的影响，这些诱导剂依赖于水杨酸（SA）和/或茉莉酸（JA）途径。为此，采用茉莉酸甲酯、噻苯隆或膦酸盐处理葡萄叶片插条。
根据诱导剂，使用 H NMR 测量和主成分分析来阐明常见和有区别的代谢物。
确定了包括碳水化合物、氨基酸、有机酸、酚类和胺类在内的广泛化合物。通过将 PC1 与 PC2 和 PC1 与 PC3 结合的得分图允许对样品进行清晰的分离，因此代谢指纹图谱显示在诱导后初级代谢途径的广泛重编程。
所应用的方法被发现非常准确，可用于根据植物样品的代谢组成进行快速测定和差异特征分析。这些研究非常有用，因为植物防御刺激物的应用作为葡萄园农药的替代策略变得越来越重要。
