Pei Zijie, Gao Mingyang, Xing Junhui, Wang Changbao, Zhao Piqian, Zhang Hongtao, Qu Jing
Department of Cell Biology, School of Biology and Basic of Medical Science, Medical College of Soochow University, Suzhou 215123, China.
Department of Orthopedics, the First Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu, China.
Int J Bioprint. 2023 Jan 5;9(2):662. doi: 10.18063/ijb.v9i2.662. eCollection 2023.
Cartilage damage is a common orthopedic disease, which can be caused by sports injury, obesity, joint wear, and aging, and cannot be repaired by itself. Surgical autologous osteochondral grafting is often required in deep osteochondral lesions to avoid the later progression of osteoarthritis. In this study, we fabricated a gelatin methacryloyl-marrow mesenchymal stem cells (GelMA-MSCs) scaffold by three-dimensional (3D) bioprinting. This bioink is capable of fast gel photocuring and spontaneous covalent cross-linking, which can maintain high viability of MSCs and provide a benign microenvironment to promote the interaction, migration, and proliferation of cells. experiments, further, proved that the 3D bioprinting scaffold can promote the regeneration of cartilage collagen fibers and have a remarkable effect on cartilage repair of rabbit cartilage injury model, which may represent a general and versatile strategy for precise engineering of cartilage regeneration system.
软骨损伤是一种常见的骨科疾病，可由运动损伤、肥胖、关节磨损和衰老引起，且自身无法修复。对于深度骨软骨损伤，通常需要进行手术自体骨软骨移植，以避免骨关节炎的后期进展。在本研究中，我们通过三维（3D）生物打印制备了明胶甲基丙烯酰-骨髓间充质干细胞（GelMA-MSCs）支架。这种生物墨水能够快速凝胶光固化和自发共价交联，可维持间充质干细胞的高活力，并提供良性微环境以促进细胞间相互作用、迁移和增殖。进一步的实验证明，3D生物打印支架可促进软骨胶原纤维再生，对兔软骨损伤模型的软骨修复有显著效果，这可能代表了一种用于软骨再生系统精确工程的通用且多功能策略。
