Tang Guiying, Xu Pingli, Ma Wenhua, Wang Fang, Liu Zhanji, Wan Shubo, Shan Lei
Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Bio-Tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, China.
College of Life Sciences, Shandong University, Jinan, China.
Front Plant Sci. 2018 Mar 6;9:260. doi: 10.3389/fpls.2018.00260. eCollection 2018.
Peanut ( L.) is one of the major oil crops and is the fifth largest source of plant oils in the world. Numerous genes participate in regulating the biosynthesis and accumulation of the storage lipids in seeds or other reservoir organs, among which several transcription factors, such as (), , and (), involved in embryo development also control the lipid reservoir in seeds. In this study, the gene was transferred into the peanut genome and expressed in a seed-specific manner driven by the NapinA full-length promoter or its truncated 230-bp promoter. Four homozygous transgenic lines, two lines with the longer promoter and the other two with the truncated one, were selected for further analysis. The mRNA level and the corresponding protein accumulation in different transgenic overexpression lines were altered, and the transgenic plants grew and developed normally without any detrimental effects on major agronomic traits. In the developing seeds of transgenic peanuts, the mRNA levels of a series of genes were upregulated. These genes are associated with fatty acid (FA) biosynthesis and lipid accumulation. The former set of genes included the homomeric ACCase A (), the BC subunit of heteromeric ACCase (), ketoacyl-ACP synthetase (), and stearoyl-ACP desaturase (), while the latter ones were the diacylglycerol acyltransferases and oleosins (, and ). The oil content and seed weight increased by 4.42-15.89% and 11.1-22.2%, respectively, and the levels of major FA components including stearic acid, oleic acid, and linoleic acid changed significantly in all different lines.
花生（Arachis hypogaea L.）是主要的油料作物之一，是世界第五大植物油来源。众多基因参与调控种子或其他储存器官中储存脂质的生物合成和积累，其中一些参与胚胎发育的转录因子，如（此处原文缺失具体转录因子名称）、（此处原文缺失具体转录因子名称）和（此处原文缺失具体转录因子名称），也控制种子中的脂质储存。在本研究中，（此处原文缺失具体基因名称）基因被转入花生基因组，并由NapinA全长启动子或其截短的230 bp启动子驱动以种子特异性方式表达。选择了四个纯合转基因株系，两个株系带有较长的启动子，另外两个带有截短的启动子，进行进一步分析。不同转基因过表达株系中（此处原文缺失具体基因名称）mRNA水平和相应蛋白质积累发生改变，转基因植株生长发育正常，对主要农艺性状没有任何不利影响。在转基因花生的发育种子中，一系列基因的mRNA水平上调。这些基因与脂肪酸（FA）生物合成和脂质积累有关。前一组基因包括同聚体乙酰辅酶A羧化酶A（此处原文缺失具体基因名称）、异聚体乙酰辅酶A羧化酶的BC亚基（此处原文缺失具体基因名称）、酮脂酰-ACP合成酶（此处原文缺失具体基因名称）和硬脂酰-ACP去饱和酶（此处原文缺失具体基因名称），而后者是二酰甘油酰基转移酶和油质蛋白（此处原文缺失具体基因名称）。油含量和种子重量分别增加了4.42 - 15.89%和11.1 - 22.2%，所有不同株系中主要脂肪酸成分包括硬脂酸、油酸和亚油酸的水平均发生了显著变化。
